Sequences from confirmation amplicon


Confirmation sequences for line 751H10 and hit At5g20690 are shown.
Line ID 751H10
Locus At5g20690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7003062
Primers used for PCR EU55 - 8474, show primer details
   
Sequence Name 57-K103582-0022-751-H10C-EU55
GenBank Accession LN509391 [GenBank]
Sequencing primer gene specific primer, EU55: 5'-CCTTTACCAATATTCGACACACCT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K103582-0022-751-H10C-EU55
CGCTTCTGCTGNATCTGTAGACCTTTTGGCGGTCGTGGATGAAGACTCGGATATCCTGAC
TTCCACAACATCGTTTTCACGGTTGTTGGCGAGCATTCTAAAGTCAGGGTTCTTCTTCTT
ATTTCGCCTCTTTATAACGCCAACAATAATAAAGAACAGGAGAAGAAGCGATATCGACAC
CATGATTGCGTTTATTGCGGCCTTGTTCGAGGTTTCTGGTACCGAGGAGGATGGTTTCGA
CGGGGGTTGTCCTTCTTGCGGATCGTTTAGCTCAATATTCTCACATCCAACGTCCACCAC
CGGCCCGCACAAGTATTCGTTCTCTGTCAAATTAACATGAAATCTACATGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTACG
CAGCGT
   
Sequence Name 73-K103583-0022-751-H10C-8409
GenBank Accession LN512879 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K103583-0022-751-H10C-8409
TAGATTTCATGTTAATTTGACAGAGAACGAATACTTGTGCGGGCCGGTGGTGGACGTTGG
ATGTGAGAATATTGAGCTAAACGATCCGCAAGAAGGACAACCCCCGTCGAAACCATCCTC
CTCGGTACCAGAAACCTCGAACAAGGCCGCAATAAACGCAATCATGGTGTCGATATCGCT
TCTTCTCCTGTTCTTTATTATTGTTGGCGTTATAAAGAGGCGAAATAAGAAGAAGAACCC
TGACTTTAGAATGCTCGCCAACAACCGTGAAAACGATGTTGTGGAAGTCAGGATATCCGA
GTCTTCATCCACGACCGCCAAAAGGTCTACAGATTCAAGCAGGAAGCGCGGTGGGCACTC
GGATGACGGTTCCACCAAGAAAGGTGTGTCGAA
Primers used for PCR 60wz - 8474, show primer details
   
Sequence Name 31-K118781-0022-751-H10C-8409
GenBank Accession LN500353 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K118781-0022-751-H10C-8409
TCATTGAATATACATGGCTGTTGGTTGAGAGGTCGAGGACTTTAAGGTTCTTCATGCTGC
CAAACTCTGGAGGTATCTCTCCGGTGAGGTTATTACTTTGCATGTGAAGCTCCTCGAGTT
GAGGGAGCTGCGTAATGGAGGAAGGGATACTTCCCTCGAACTTGTTATGATCAAGAAATA
GTCGCTTGAGCTTTGACATGTCCTTGAAAAAATCGTCACGGATCTCTCCGGAGAAGCTGT
TGTTAGAGAGCATGAGAGATTTTAGGCCACGGAGTTTAAAGAAATGCGGGAGAGGACCGG
AGAGGAGGTTGTTGTCAAGCCTGATCGTCTTTAGATTTGGGAGATCCTTTAAATCATCCA
CGGTGATGGTTCCTGAGAGACCTAGACGTGTGACGTGAATGCCTGAGACTGTTAGACCCT
TTTGGCAATAGATCCCAAACCATTTCCCACTGCAAGGATCTGTCCCTTCTCTCCATGAAT
TTAGGTCCCCTTTGGTTATCTTCACCGAGTTTTTGAACCGCACAAGCGGTTCGGACTCGC
TCACATACTGCAAAGAAGGGGAGATTGAGAAAGATAAGAGGAGGATGAGGAGAAAGAAAC
CGGGATTCAGAACAGCAGCAGCCATGGATTGAAGAGAGGAGATGCTCTTTTTCTTTTTCT
TGTTCTTGTTGGGATGTTACGTCTTAAAACAAGGACAATGTTTTTGATAATGATGATGAT
GCTCTATAGTTTGTTGAAAGGTGTGGTTTTGTTTAGATTGTGTTTGTTGTTCCAAACTTT
GGACATTGTCTGTTTGTGAAGGTTGAGAAGGAAAGGGTGGCATTTTTTCTTTGATTGAAT
TAGTTGTCAAAGTTTAGACTTTTTTTTTAGCAAAAATAGTGTTTTTGTTATATTTTTTGT
ATCCTATTTGCTTGTTT
   
Sequence Name 71-K118782-0022-751-H10C-60wz
GenBank Accession LN512638 [GenBank]
Sequencing primer gene specific primer, 60wz: 5'-CCTATTCGATTTGTCCGAATTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K118782-0022-751-H10C-60wz
TGAGACATTTACCTGTTGAAAAGAAATAGATAGAGATGCAAATGTATAAGATTAAACTTC
AAGATCCTTATCAAAAAATTCTCAAAGCTCTCAGAAACAAGCAAATAGGATTACAAAAAT
TATAACAAAAACACTATTTTTGGCTTAAAAAAAAGTCTAAACTTTGACAACTAATTCAAT
CAAAGAAAAAATGCCACCCTTTCCTTCTCAACCTTCACAAACAGACAATGTCCAAAGTTT
GGAACAACAAACACAATCTAAACAAAACCACACCTTTCAACAAACTATAGAGCATCATCA
TCATTATCAAAAACATTGTCCTTGTTTTAAGACGTAACATCCCAACAAGAACAAGAAAAA
GAAAAAGAGCATCTCCTCTCTTCAATCCATGGCTGCTGCTGTTCTGAATCCCGGTTTCTT
TCTCCTCATCCTCCTCTTATCTTTCTCAATCTCCCCTTCTTTGCAGTATGTGAGCGAGTC
CGAACCGCTTGTGCGGTTCAAAAACTCGGTGAAGATAACCAAAGGGGACCTAAATTCATG
GAGAGAAGGGACAGATCCTTGCAGTGGGAAATGGTTTGGGATCTATTGCCAAAAGGGTCT
AACAGTCTCAGGCATTCACGTCACACGTCTAGGTCTCTCAGGAACCATCACCGTGGATGA
TTTAAAGGATCTCCCAAATCTAAAGACGATCAGGCTTGACAACAACCTCCTCTCCGGTCC
TCTCCCGCATTTCTTTAAACTCCGTGGCCTAAAATCTCTCATGCTCTCTAACAACAGCTT
CTCCGGAGAGATCCGTGACGATNTTTTCAAGGACATGTCAAAGCTCANGCGACTATNTCT
TGATCATAAACAGTTCGAGGGAAGTATCCCTTCCTCCCATACGCAGCTCCCTCAACTCGA
GGAGCTTCACA

Last Updated on 06.12.2018 16:11