Sequences from confirmation amplicon


Confirmation sequences for line 752H04 and hit At3g15890 are shown.
Line ID 752H04
Locus At3g15890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5374681
Primers used for PCR 70ns - 8474, show primer details
   
Sequence Name 81-K114626-0022-752-H04C-70ns
GenBank Accession LN514099 [GenBank]
Sequencing primer gene specific primer, 70ns: 5'-AACACTCAACAAATTCTTGTGACG-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K114626-0022-752-H04C-70ns
AGCAAATCTATCTCTTCTCTATTACTCCATTCCTTCAACCTCTTGACTGCAATCTGTAAA
AAGACAAAATAATCAACCTCACAAGATCTCCAGTTTGAACAAGAAGCAAGTCCTAGATAA
CATGTTGAGGTCAAATTACTTGTGAGCCATCCCATAGCTGACCCCAATAGACACTACCAA
ATCGACCTTCACCGAGTTTGTTATCGTAATTGAAACTGTTGGTGGCTGCATGAAGTTCCT
TCAGTGAAAATACCCTCCAAGATGGCTCTTCCTTTTCTTTCTTCTTCCTGTGTAATTTAA
TCTCAGTATCCTCAAAAACTTATATTTAGATCATTCATCAACACATTAACAAACAAATAA
CTTATGAAACGCAGAACAACAGTTGAATCAATTATCCCTTGCAAAACCATGTCTTTAGAA
GGAATAAGACCACCCTGAAACTACTCAAATTGCTATTAATGATGAAACTTTCAAGGATCA
ATCAGGTGATATATTATATAGTGAGGATGATAAAAAAAAGAACAGATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCC
   
Sequence Name 81-K114625-0022-752-H04C-8409
GenBank Accession LN514098 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K114625-0022-752-H04C-8409
TTTTTTTATCATCCTCACTATATAATATATCACCTGATTGATCCTTGCAAGTTTCATCAT
TAATAGCAATTTGAGTAGTTTCAGGGTGGTCTTATTCCTTCTAAAGACATGGTTTTGCAA
GGGATAATTGATTCAACTGTTGTTCTGCGTTTCATAAGTTATTTGTTTGTTAATGTGTTG
ATGAATGATCTAAATATAAGTTTTTGAGGATACTGAGATTAAATTACACAGGAAGAAGAA
AGAAAAGGAAGAGCCATCTTGGAGGGTATTTTCACTGAAGGAACTTCATGCAGCCACCAA
CAGTTTCAATTACGATAACAAACTCGGTGAAGGTCGATTTGGTAGTGTCTATTGGGGTCA
GCTATGGGATGGCTCACAAGTAATTTGACCTCAACATGTTATCTAGGACTTGCTTCTTGT
TCAAACTGGAGATCTTGTGAGGTTGATTATTTTGTCTTTTTACAGATTGCAGTCAAGAGG
TTGAAGGAATGGAGTAATAGAGAAGAGATAGATTTTGCTGTGGAAGTCGAGATTCTTGCT
CGTATTCGTCACAAGAATTTGTGGAGTG

Last Updated on 06.12.2018 16:11