Sequences from confirmation amplicon


Confirmation sequences for line 753B02 and hit At5g38690 are shown.
Line ID 753B02
Locus At5g38690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15480543
Primers used for PCR CR86 - 8474, show primer details
   
Sequence Name 54-K107248-0022-753-B02C-CR86
GenBank Accession LN508496 [GenBank]
Sequencing primer gene specific primer, CR86: 5'-ATGTTATCTGGGAGAAGCAAAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K107248-0022-753-B02C-CR86
TCTCCCAGATGATATCCACTACTTACTGTGATATTAGAGGATAGAGGAGAAACGTAAGAA
TAAGTTTTCTTAAAAAGTATTATGTTTTCTGTCAGCTTCCTTCTCTGATGATGCATGTCT
GATTCATTTCCATTTTAAAATAATATAGATCCGTTTGCCTGAGTGCAACTGATCCTAGTT
GGTTCACTACGATTGGAGAATGTCTCTCGGAATCAGAAGTAAAGCTAGATGATTTCCCTC
CTGAAATGTTTGAAAAAGGCATTTCTCAGTATGAGAAGTTGAATTCATCAAAAAGGCTTA
AGCTTTTGAATTTCTTATGCGATGAAACGCTTGGCACATTGTAAGTAATAGTTATACTGA
ATCAACTACTATCTTTGATTTAAGGTACTTGAGGGTTTACATATTGCGATAACATTTTCA
GGGTGATGAGGAATTGCATTGATAGCCAAAATATTGAATCTGTTGAAAGGAAAAAAGAAG
CAAAAGAAAAGATCAATGCTGCAAAAGATAAGGTAACTCTAAGATCAACAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GC
   
Sequence Name 38-K107247-0022-753-B02C-8409
GenBank Accession LN503001 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K107247-0022-753-B02C-8409
GAGCCATTTACAATTGAATATATCCTGTTGATCTTAGAGTTACCTTATCTTTTGCAGCAT
TGATCTTTTCTTTTGCTTCTTTTTTCCTTTCAACAGATTCAATATTTTGGCTATCAATGC
AATTCCTCATCACCCTGAAAATGTTATCGCAATATGTAAACCCTCAAGTACCTTAAATCA
AAGATAGTAGTTGATTCAGTATAACTATTACTTACAATGTGCCAAGCGTTTCATCGCATA
AGAAATTCAAAAGCTTAAGCCTTTTTGATGAATTCAACTTCTCATACTGAGAAATGCCTT
TTTCAAACATTTCAGGAGGGAAATCATCTAGCTTTACTTCTGATTCCGAGAGACATTCTC
CAATCGTAGTGAACCAACTAGGATCAGTTGCACTCAGGCAAACGGATCTATATTATTTTA
AAATGGAAATGAATCAGACATGCATCATCAGAGAAGGAAGCTGACAGAAAACATAATACT
TTTTAAGAAAACTTATTCTTACGTTTCTCCTCTATCCTCTAATATCACAGTAAGTAGTTG
GATTATCATCTGTGTGAGAGTGGAATATTGTTGTCTTCTTTTGCTTCTCCCGAATAACA

Last Updated on 06.12.2018 16:11