Sequences from confirmation amplicon


Confirmation sequences for line 754F03 and hit At4g00940 are shown.
Line ID 754F03
Locus At4g00940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:403962
Primers used for PCR BM80 - 8474, show primer details
   
Sequence Name 37-K104418-0022-754-F03C-BM80
GenBank Accession LN502605 [GenBank]
Sequencing primer gene specific primer, BM80: 5'-TTGTTCCCACTCTCTCAGATCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K104418-0022-754-F03C-BM80
TCTTCTTATCTATACTATATAGTTCCATGTATAGATATTCTCTATAGTTTTTGTTTCATG
GACTTCAACTTTTTATGTTCTCTCAATAAGTTTTATTAATTTACTTATTTATTTGTATGT
GCTACTTTTTATTAGATCCACTTTTGCTAGCATGCCTTTATATGCTTAATTCAATGGTTT
GATCATACGTATACTTATTACAGGGATTAATTACTGTAATTGAAGCTCACATCATGGACC
ATCATCAGTATCATCATCATGATCAATACCAACATCAGATGATGACTAGTACTAACAATA
ATTCCTATAACACCATCGTCACAACACAACCACCACCAACAACAACAACAATGGATTCAA
CAACAGCAACAACTATGATAATGGATGACGAGAAGAAGTTGATGACGACAATGAGCACTA
GGCCGCAAGAACCAAGAAACTGTCCAAGATGCAACTCAAGCAACACCAAGTTTTGTCGGA
GATATTGGACTGAAGGTGGCTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCA
   
Sequence Name 53-K104419-0022-754-F03C-8409
GenBank Accession LN508146 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K104419-0022-754-F03C-8409
AGCCACCTTCAGTCAATATCTCCGACAAAACTTGGTGTTGCTTGAGTTGCATCTTGGACA
GTTTCTTGGTTCTTGCGGCCTAGTGCTCATTGTCGTCATCAACTTCTTCTCGTCATCCAT
TATCATAGTTGTTGCTGTTGTTGAATCCATTGTTGTTGTTGTTGGTGGTGGTTGTGTTGT
GACGATGGTGTTATAGGAATTATTGTTAGTACTAGTCATCATCTGATGTTGGTATTGATC
ATGATGATGATACTGATGATGGTCCATGATGTGAGCTTCAATTACAGTAATTAATCCCTG
TAATAAGTATACGTATGATCAAACCATTGAATTAAGCATATAAAGGCATGCTAGCAAAAG
TGGATCTAATAAAAAGTAGCACATACAAATAAATAAGTAAATTAATAAAACTTATTGAGA
GAACATAAAAAGTTGAAGTCCATGAAACAAAAACTATAGAGAATATCTATACATGGAACT
ATATATGTATAAGATAAGAAGAATCTAGATTAATCAAGAAAAGATCTGAGAGAGTGGGAA
CA

Last Updated on 06.12.2018 16:11