Sequences from confirmation amplicon


Confirmation sequences for line 754F06 and hit At1g61240 are shown.
Line ID 754F06
Locus At1g61240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22584227
Primers used for PCR 16dh - 8474, show primer details
   
Sequence Name 57-K109676-0022-754-F06C-16dh
GenBank Accession LN509444 [GenBank]
Sequencing primer gene specific primer, 16dh: 5'-AACACACACATTACACTCCTGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K109676-0022-754-F06C-16dh
AGCAGATTCTCATCAGGTAATAATAACTCACCTCTGTTCTTGAATCAAAAGTCGCAGAAC
CGCGTCTCTGTACATAAGAAAAAAGTGACCAAAGTTGTGAACTTTCTGTTTGTTTTACAG
TAAACAAATGAGGATTTGGATAGTTACGTAGCTTACCCTATTTACTCCGCTGCGAGCTGA
GTTCTGTGGAAATTAGGAAGAGATTATTCGTTTATCGGTTTTGGGTATACATACAAGGAG
AATAGGTGGGGAAGTAGATGATCTTACCTTCTTATCGGGATAACCGCTTCCACCTAGTGT
TTGAATGCCTTGATGGAAGATATATTCACTGTCGACAATGCCTACTTTCTTGCTACGGTC
ACCCTAAAAACGACATTTTCAAAACCGATTCATCTAGAGAATGCAATCGGATCACAATGC
TAATGGAGACGAGTGATGTTGCAAGGTCTTAAAGAAGAAAAAGTCTTTACCTGAGCACAG
TATCCAAGTTTCATATCCATTCCCCAACCATGAACCAGATCATTCTACAAACCGCANAAA
CAATAAGTAGGATGCAATTTCATTTAAAGAAGAACAGATATACATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 41-K109675-0022-754-F06C-8409
GenBank Accession LN504108 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K109675-0022-754-F06C-8409
CTGTTCTTCTTTAATGAATTGCATCCTACTTATTGTTTTTGCGGTTTGTAGAATGATCTG
GTTCATGGTTGGGGAATGGATATGAAACTTGGATACTGTGCTCAGGTAAAGACTTTTTCT
TCTTTAAGACCTTGCAACATCACTCGTCTCCATTAGCATTGTGATCCGATTGCATTCTCT
AGATGAATCGGTTTTGAAAATGTCGTTTTTAGGGTGACCGTAGCAAGAAAGTAGGCATTG
TCGACAGTGAATATATCTTCCATCAAGGCATTCAAACACTAGGTGGAAGCGGTTATCCCG
ATAAGAAGGTAAGATCATCTACTTCCCCACCTATTCTCCTTGTATGTATACCCAAAACCG
ATAAACGAATAATCTCTTCCTAATTTCCACAGAACTCAGCTCGCAGCGGAGTAAATAGGG
TAAGCTACGTAACTATCCAAATCCTCATTTGTTTACTGTAAAACAAACAGAAAGTTCACA
ACTTTGGTCACTTTTTTCTTATGTACAGAGACGCGGTTCTGCGACTTTTGATTCAAGAAC
AGAGGTGAGTTATTATTACCTGATGATGAATCTGCTTTAGGAACTTTCTTTCGTCTATAG
AGCAGGAGTGT

Last Updated on 06.12.2018 16:11