Sequences from confirmation amplicon


Confirmation sequences for line 754G01 and hit At5g65630 are shown.
Line ID 754G01
Locus At5g65630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26227047
Primers used for PCR 19kn - 8474, show primer details
   
Sequence Name 89-K113669-0022-754-G01C-19kn
GenBank Accession LN514971 [GenBank]
Sequencing primer gene specific primer, 19kn: 5'-CACAGACGAAGAAAACAATTTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K113669-0022-754-G01C-19kn
GTTTGATGGAAGGAGTGCAAATTTGCTTACCATGTTCCTAGGTGGAGTTGACACGTTCCT
GATAAACCCTTGGCGCTTGATTTTGCTAGCCATCTTCTTGTAGTTCGTCACAAAACGATC
AAGCTCCCATAGAGTCTCATTGTCGACAGCTTCTATATCCAGCTCAATTTCGTCCCCATC
TTGAGCTAAATGTCCGTTTCTCTTCCTGAGAATCTGAAGCAACTGACCAAGCTTCTCAGG
AGGCAAGTCTTGTAGATTCATACCAAGCTTTGACTTCTCTTCCATCGTCAAATGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTACCGCAGCGTTATT
   
Sequence Name 58-K113666-0022-754-G01C-8409
GenBank Accession LN509762 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K113666-0022-754-G01C-8409
TTGACGATGGAAGAGAAGTCAAAGCTTGGTATGAATCTACAAGACTTGCCTCCTGAGAAG
CTTGGTCAGTTGCTTCAGATTCTCAGGAAGAGAAACGGACATTTAGCTCAAGATGGGGAC
GAAATTGAGCTGGATATAGAAGCTGTCGACAATGAGACTCTATGGGAGCTTGATCGTTTT
GTGACGAACTACAAGAAGATGGCTAGCAAAATCAAGCGCCAAGGGTTTATCAGGAACGTG
TCAACTCCACCTAGGAACATGGTAAGCAAATTTGCACTGCCTTCCTATCAAAACCACCAT
TTGATCACTGTGGTTTCAATACTCAAAATTGTTTTCTTCGTCTGTG

Last Updated on 06.12.2018 16:11