Sequences from confirmation amplicon


Confirmation sequences for line 756D02 and hit At3g58450 are shown.
Line ID 756D02
Locus At3g58450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21622359
Primers used for PCR 17mh - 8474, show primer details
   
Sequence Name 55-K114180-0022-756-D02C-17mh
GenBank Accession LN508879 [GenBank]
Sequencing primer gene specific primer, 17mh: 5'-AATGCCCTGTATATCTCAAACACC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K114180-0022-756-D02C-17mh
CACCGAATCTCATTTGTTGTAGACCTTAGACCAAAAATGAAACTTGTACCTTTTGATCAT
GCCGAGTCCACGGCTACCAACAACAAGAAGATCAACATGTGTTTGCTCGACGGCTTGGCA
TATCATCTCCTTAGGATCTCCTTCCAATATCATAGTCTCTGTCTTCACCTACAACATAAA
ACCATAAACTCAAGAGACAGAGAAGATCACTGATAAACCTAAACTCTTATGAAACCTTGA
TAAACCTAAACTGAAAGTAAACTTCATAGACCCTAAACTTATTGTATTTTTGAAATTTTT
TCCAAACTTGTAGTATGTTGAAAGTTGAAACTAGACATGTCTTGATGGTAATTTTTAGGA
AATTATTTCACACATATAGCGCACCATTTTGCCTCGGCATATCTCCAATGCCCGTGTGAA
TAAGTTTGTCGTACTCTCTTCTCGTGCTTTCCTCATTGGTTCGGGCACTGAATCTGTCGC
ATACACCGCCGAAGCTAAGAATTTCCAAACTCACTCAATATTGTATACAGTCACATCCCC
GGATATATTCCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 19-K114177-0022-756-D02C-8409
GenBank Accession LN494852 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K114177-0022-756-D02C-8409
TTTCATTGAATTATCCTGGGAATAATCCGGGGATGTGACTGTATACATATTGAGTGAGTT
TGGAAATTCTTAGCTTCGGCGGTGTATGCGACAGATTCAGTGCCCGAACCAATGAGGAAA
GCACGAGAAGAGAGTACGACAAACTTATTCACACGGGCATTGGAGATATGCCGAGGCAAA
ATGGTGCGCTATATGTGTGAAATAATTTCCTAAAAATTACCATCAAGACATGTCTAGTTT
CAACTTTCAACATACTACAAGTTTGGAAAAAATTTCAAAAATACAATAAGTTTAGGGTCT
ATGAAGTTTACTTTCAGTTTAGGTTTATCAAGGTTTCATAAGAGTTTAGGTTTATCAGTG
ATCTTCTCTGTCTCTTGAGTTTATGGTTTTATGTTGTAGGTGAAGACAGAGACTATGATA
TTGGAAGGAGATCCTAAGGAGATGATATGCCAAGCCGTCGAGCAAACACATGTTGATCTT
CTTGTTGTTGGTAGCCGTGGACTCGGCATGATCAAAAGGTACAAGTTTCATTTTTGGTCT
AAGGTCTAACAACAAATTGAGATTTCTGGTTGTGACTATTCATTCATTCCTGGTGTTTGA
GAT

Last Updated on 06.12.2018 16:11