Sequences from confirmation amplicon


Confirmation sequences for line 756G08 and hit At3g13670 are shown.
Line ID 756G08
Locus At3g13670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4471296
Primers used for PCR HC02 - 8474, show primer details
   
Sequence Name 43-K027154-022-756-G08C-8409
GenBank Accession LN504713 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K027154-022-756-G08C-8409
GTAGATTTCCCGGACATGAAGCCATTTTTGTTGAATCTCTCTCTTCTTAATTTTTATAGA
GGGACAGTTAGATACGCAAGTGCACATGCTCATTTAGGGAGAACTGCTAGCAGAAGGGAT
GATCTGGAATCATTAGCATATACACTGATCTTTCTTCACCGAGGTAGATTGCCATGGCAA
GGATATCAGGTACTGCAGACCTATCATTTTGTTTTGTAGAATCATGTTGTTGCATGATTC
TTTCTCTGCTAATCCTGTCTCTTACTCAATTTTCAGGGAGATAACAAGTCATTCCTTGTT
TGCAAAAAGAAGATGGCAACATCGCCGGATATGCTTTGTTGTTTTTGTCCCCCTCCTTTC
AAACA
   
Sequence Name 47-K027154-022-756-G08C-HC02
GenBank Accession LN506078 [GenBank]
Sequencing primer gene specific primer, HC02: 5'-CTCAAGAAATTGTTTGAAAGGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K027154-022-756-G08C-HC02
CAAAAACAACAAAGCATATCCGGCGATGTTGCCATCTTCTTTTTGCAAACAAGGAATGAC
TTGTTATCTCCCTGAAAATTGAGTAAGAGACAGGATTAGCAGAGAAAGAATCATGCAACA
ACATGATTCTACAAAACAAAATGATAGGTCTGCAGTACCTGATATCCTTGCCATGGCAAT
CTACCTCGGTGAAGAAAGATCAGTGTATATGCTAATGATTCCAGATCATCCCTTCTGCTA
GCAGTTCTCCCTAAATGAGCATGTGCACTTGCGTATCTAACTGTCCCTCTATAAAAATTA
AGAAGAGAGAGATTCAACAAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATTTCGACGTGTCTACAT
Primers used for PCR HC01 - 8474, show primer details
   
Sequence Name 18-K027155-022-756-G08C-8409
GenBank Accession LN494157 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K027155-022-756-G08C-8409
GTAGATTTCCCGGACATGAAGCCATGTAGAGCGAGTATAAAACAAATTATATTAGGTAAC
AGTAGAAAATATATTACCTAAACATATCAGGACGTTGATCATATTCAACATGTTGTCCGC
TACCACCTTCTCTCCACTTTGTAGCTGAAAAGTTGGTTGAAAAATGAGAAAGGTAAAGAA
GAACCTAGCAAGAATAACAAAGAGAATGAAATCAACCTAGTCCCAAATCAACAAGAAACA
GTTTTTTCTCTTGAGACGTTGAAGGCTGGCCGAGTAAGAAATTTTCTGGCTTAACATCCC
CATGCACATAACTGAAACCGAAGTAAAACATGACCTGTTAGGCTAGTTAATAGAGAGAAA
ACATAAGAACTT
   
Sequence Name 22-K027155-022-756-G08C-HC01
GenBank Accession LN495964 [GenBank]
Sequencing primer gene specific primer, HC01: 5'-TCGGCACCTCTAAGTTCTTATGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K027155-022-756-G08C-HC01
CTCTCTATTAACTAGCCTAACAGGTCATGTTTTACTTCGGTTTCAGTTATGTGCATGGGG
ATGTTAAGCCAGAAAATTTCTTACTCGGCCAGCCTTCAACGTCTCAAGAGAAAAAACTGT
TTCTTGTTGATTTGGGACTAGGTTGATTTCATTCTCTTTGTTATTCTTGCTAGGTTCTTC
TTTACCTTTCTCATTTTTCAACCAACTTTTCAGCTACAAAGTGGAGAGAAGGTGGTAGCG
GACAACATGTTGAATATGATCAACGTCCTGATATGTTTAGGTAATATATTTTCTACTGTT
ACCTAATATAATTTGTTTTATACTCGCTCTACATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT
CGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11