Sequences from confirmation amplicon


Confirmation sequences for line 757B03 and hit At5g60320 are shown.
Line ID 757B03
Locus At5g60320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24271218
Primers used for PCR EU56 - 8474, show primer details
   
Sequence Name 59-K103693-0022-757-B03C-EU56
GenBank Accession LN509979 [GenBank]
Sequencing primer gene specific primer, EU56: 5'-GAAACTCCAGAAACAAAACCAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K103693-0022-757-B03C-EU56
CATGGCTTCTGAAGATTGCATCTGATCTTATTGGTCTTCTTTAATCATCTGACTTTCTTG
TTAAGTCAACAAGAAGAAGCCGGTTTCATCTACAATGGCTTTGGCCAAGCTCAAGCTGGT
CTTCATCTAGACGGAGCTGCTAAAATCCTCTTTCCAGATGGATTGTTGCAGTTGACAAAT
GCCTCAACACAACAAATGGGTCATGCTTTCTTCAAGAAACCATTCAAGTTTGATTCATAT
GAGAAGAAACTCTCTTTTTCGACTCATTTTGTGTGTGCGCTTGTGCCTAAACCGGGAGCT
GATGGTGGCCATGGAATTGCCTTTGTTGTATCCTCTTCCATAGATTTCACACAAGCAGAT
CCAACTCAGTACTTGGGACTTTTAAACATCTCAACAAATCTACATGTAGATTATTCATGC
ATGTCCGGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGAT
   
Sequence Name 75-K103694-0022-757-B03C-8409
GenBank Accession LN513228 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K103694-0022-757-B03C-8409
GCCTTTACATTGAATATATCCCGGACATGCATGAATAATCTACATGTAGATTTGTTGAGA
TGTTTAAAAGTCCCAAGTACTGAGTTGGATCTGCTTGTGTGAAATCTATGGAAGAGGATA
CAACAAAGGCAATTCCATGGCCACCATCAGCTCCCGGTTTAGGCACAAGCGCACACACAA
AATGAGTCGAAAAAGAGAGTTTCTTCTCATATGAATCAAACTTGAATGGTTTCTTGAAGA
AAGCATGACCCATTTGTTGTGTTGAGGCATTTGTCAACTGCAACAATCCATCTGGAAAGA
GGATTTTAGCAGCTCCGTCTAGATGAAGACCAGCTTGAGCTTGGCCAAAGCCATTGTAGA
TGAAACCGGCTTCTTCTTGTTGACTTAACAAGAAAGTCAGATGATTAAAGAAGACCAATA
AGATCAGATGCAATCTTTCAGAAGCCATTGCTAATGAAACAGAGAGAAGTCTGGTTTTG
Primers used for PCR BH04 - 8474, show primer details
   
Sequence Name 53-K118446-0022-757-B03C-BH04
GenBank Accession LN508300 [GenBank]
Sequencing primer gene specific primer, BH04: 5'-GATGAGGAATCTGAGGAAGTTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K118446-0022-757-B03C-BH04
CTCCATCCTAAGATATATTGGTAACTGACTAATGTTCCTGTTGCTGCAGAAAATCCAAAA
AACAGCTTTCGATCCGGGAAGATGTCAGTGAGATTAATGGATCTTGACAGAAGAGGATGG
TTTGGTTTCTGGATACTTAGAGGAGCCACTGTGACATTGAGTAAAGCTCCTTCATAATCC
ACCCAGATTTGTATAGGGTCTCCACTTAAGAGCTTGATGCTTTGATTCTTCCCTTTGGCG
TTTGAAAAGTATGAAGCTGAAGCAGACTCTACAGAATTCAGGCTTTTGATGTCTATACCG
ACATGATTCTTGTCGATATCATCAAACTCTGCGCTCTCAACAGTATCGAGCTCAATAGCT
AGAAGCTGAGAAAAAGAAAGAGTAATTACCAATTGAAATGACAATCTCCACTTGAACAGT
ATCCTGCATCAGATTGTATGAAGCCATTTCATACAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 13-K118445-0022-757-B03C-8409
GenBank Accession LN492256 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K118445-0022-757-B03C-8409
TCATTGTATGAATGGCTTCATACATCTGATGCAGGATACTGTTCAAGTGGAGATTGTCAT
TTCAATTGGTAATTACTCTTTCTTTTTCTCAGCTTCTAGCTATTGAGCTCGATACTGTTG
AGAGCGCAGAGTTTGATGATATCGACAAGAATCATGTCGGTATAGACATCAAAAGCCTGA
ATTCTGTAGAGTCTGCTTCAGCTTCATACTTTTCAAACGCCAAAGGGAAGAATCAAAGCA
TCAAGCTCTTAAGTGGAGACCCTATACAAATCTGGGTGGATTATGAAGGAGCTTTACTCA
ATGTCACAGTGGCTCCTCTAAGTATCCAGAAACCAAACCATCCTCTTCTGTCAAGATCCA
TTAATCTCACTGACATCTTCCCGGATCGAAAGCTGTTTTTTGGATTTTCTGCAGCAACAG
GAACATTAGTCAGTTACCAATATATCTTAGGATGGAGTTTCAGCAGAAGCAGAATGTTGT
TGCAGAGTCTGGACTTCTCAAAACTTCCTCAGATTTCCTCATC

Last Updated on 06.12.2018 16:11