Sequences from confirmation amplicon


Confirmation sequences for line 757B08 and hit At5g58680 are shown.
Line ID 757B08
Locus At5g58680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23708292
Primers used for PCR 04im - 8474, show primer details
   
Sequence Name 22-K112877-0022-757-B08C-8409
GenBank Accession LN496190 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K112877-0022-757-B08C-8409
TCGGATGAGCCATTTCATTGAATTTGAGAATGTTGTAAACTAAAGTGTTATCTCTCATAC
GGTTTAATGCATACTCATTGTTGTCTGGGTTGCCTAAGAAGCTCTATCAACGCCTCAGCC
TTTACCTTAGCACCACGACTCGCACTACCCTGCGATAAAGCCACGAGCGGAGGAACCGCT
CCTTCACGAGCCACCATGGTTCGATACACAACACTCTCCTCACAAAGCTGCAACAATATC
GACACACTTATCTCCTTCTGTCTCTGTGTTCCCGCTTCAACAATCTCAACCAACACCGGA
ACTCCTCCTTCTTCCACAACCGCCGGCTTCGATTCCGGCGCCGACATTAACAGATTCATA
ACAAACGCTGATTTATCAACCATATCCGATTCAAAATCAATCATTAACTCCACTAACGGC
TTCATAATCCCAGATTCAACAGCTCTGGTTTTTGTTC
   
Sequence Name 59-K112878-0022-757-B08C-04im
GenBank Accession LN510046 [GenBank]
Sequencing primer gene specific primer, 04im: 5'-AGAACAAAACCAGAGCTGTTGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K112878-0022-757-B08C-04im
TTTTGATCGGATATGGTTGATAATCAGCGTTTGTTATGAATCTGTTAATGTCGGCGCCGG
AATCGAAGCCGGCGGTTGTGGAAGAAGGAGGAGTTCCGGTGTTGGTTGAGATTGTTGAAG
CGGGAACACAGAGACAGAAGGAGATAAGTGTGTCGATATTGTTGCAGCTTTGTGAGGAGA
GTGTTGTGTATCGAACCATGGTGGCTCGTGAAGGAGCGGTTCCTCCGCTCGTGGCTTTAT
CGCAGGGTAGTGCGAGTCGTGGTGCTAAGGTAAAGGCTGAGGCGTTGATAGAGCTTCTTA
GGCAACCCAGACAACAATGAGTATGCATTAAACCGTATGAGAGATAACACTTTAGTTTTA
CAACATTCTCAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAAGGTTATTA

Last Updated on 06.12.2018 16:11