Sequences from confirmation amplicon


Confirmation sequences for line 758B02 and hit At1g64860 are shown.
Line ID 758B02
Locus At1g64860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24100476
Primers used for PCR IM06 - 8474, show primer details
   
Sequence Name 09-K032517-022-758-B02C-8409
GenBank Accession LN490065 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K032517-022-758-B02C-8409
TTACAAGAGTATTGCACTTATAAGCTTGCTTACTTCCTCCTGCAATTTTATCATCAATCT
CATATGCTCAAACTCTAGTGTTTCATGATAGAGAAAATCAGAACCATTGTATTAAATGTA
GCTTCACTTATGACTTGTTCTTTTTTTTTTTTTACCTTGAGGGCCAAATCATCATACCCG
TGCCACGGATTGTTCTCCAAACGAGTATCCCCAATGTACTGGAAATAGTGAAACAAACAA
GCAAATCGTTATAAACTGGAAAGGAAAAACCCATGGAAACTTGGATGGGGAAAACAAAAT
CGTCGTTTTGTTTTAAAAAAAACCGAAAAAAAAACTCCCACTGTGATGAGTTTCTCCAGG
GAGACCATTCAAAAAAGGAAATGCATCTCTGTCTAAAAAAAATACTTTGCTTACACCCTG
TATTTTGCAAATGCAAAAAAGTAAGCACAAATACACATAGATCCCCACGTCNNTGTCT
   
Sequence Name 09-K032517-022-758-B02C-IM06
GenBank Accession LN490066 [GenBank]
Sequencing primer gene specific primer, IM06: 5'-GGGGATCTATGTGTATTTGTGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K032517-022-758-B02C-IM06
TTTTTCCGTTTCTCCTAAACAAAACGACGATTTTGTTTTCACCATCCATGTTTCCATGTG
TCTCTCCTCTCCAGTTTATAACGATTTGCTTGTTTGTTTCACTATTTCCAGTACATTGCG
GATACTCGTTTGGAGAACAATCCGTGGCACGGGTATGATGATTTGGCACTCAAGGTAAAA
AAAAAAAAAGAACAAGTCATAAGTGAAGCCACCTTTAATACAAGGGTTCTGATTTTCTCT
ATCTTGAAACACTAGAGTTTGAGCATATGAGATTGATGATAAAATTGCAGGAGGAAGTAA
GCAAGCTTATAAGTGCAATACTCTTTGTAAATGGCTTCCTGTCCGGGAAATCTACATGGA
TCACCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAAATGTCCGCCGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACAATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11