Sequences from confirmation amplicon


Confirmation sequences for line 758E01 and hit At3g09440 are shown.
Line ID 758E01
Locus At3g09440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2903748
Primers used for PCR JP14 - 8474, show primer details
   
Sequence Name 37-K027065-022-758-E01C-8409
GenBank Accession LN502483 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K027065-022-758-E01C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGAAATGCTCTTGAGAATTA
CGCATACAACATGAGGAACACAATCCGTGATGAGAAGATTGGCGAGAAGCTTGCTGGAGA
TGACAAGAAGAAGATTGAAGACTCAATTGAAGCAGCCATTGAATGGCTAGAAGCGAATCA
GCTAGCAGAGTGTGATGAATTTGAGGACAAGATGAAAGAGCTCGAGAGTATTTGCAACCC
GATCATAGCAAAGATGTACCAAGGCGGTGAAGCTGGTGGTCCAGCTGCAGGAGGAATGGA
CGAGGATGTTCCTCCTTCTGCCGGAGGTGCAGGTCCCAAGATTGAGGAAGTCGACTAATA
ACTTTCTCTCTTACTCTCTTACTCTCAGTCTTTATGTGTTTGTATTTCAACATTTTCCTG
TTTTG
   
Sequence Name 38-K027065-022-758-E01C-JP14
GenBank Accession LN502856 [GenBank]
Sequencing primer gene specific primer, JP14: 5'-AACTAGGGGACAAAACAGGAAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K027065-022-758-E01C-JP14
AAATACAAACACATAAAGACTGAGAGTAAGAGAGTAAGAGAGAAAGTTATTAGTCGACTT
CCTCAATCTTGGGACCTGCACCTCCGGCAGAAGGAGGAACATCCTCGTCCATTCCTCCTG
CAGCTGGACCACCAGCTTCACCGCCTTGGTACATCTTTGCTATGATCGGGTTGCAAATAC
TCTCGAGCTCTTTCATCTTGTCCTCAAATTCATCACACTCTGCTAGCTGATTCGCTTCTA
GCCATTCAATGGCTGCTTCAATTGAGTCTTCAATCTTCTTCTTGTCATCTCCAGCAAGCT
TCTCGCCAATCTTCTCATCACGGATTGTGTTCCTCATGTTGTATGCGTAATTCTCAAGAG
CATTTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACG

Last Updated on 06.12.2018 16:11