Sequences from confirmation amplicon


Confirmation sequences for line 759C08 and hit At5g17850 are shown.
Line ID 759C08
Locus At5g17850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5899527
Primers used for PCR HP48 - 8474, show primer details
   
Sequence Name 84-K033555-022-759-C08C-8409
GenBank Accession LN514376 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K033555-022-759-C08C-8409
ATTTACAATTGAATAATCCTGTTCATATTGATCCATGTAGTTTCTTCTCTTCCTCTGGTT
GCTTCTCTTGTTCTATCTATTGGGTCATACGGCCTCTGAGTACTTTTGTTCATCTCTTGA
AAGTCTCTCAAAGCTGCTTAATTTGTCACCAACTGTTGCTGGTGTTACTCTTTTGTCGCT
TGGTAATGGTGCTCCTGATTTGTTTGCAAGTTTGGTCTCTTTCATGGGAGAATCTAAAGG
TACTTATGATGTTGGTCTCAACACTGTTGTGGGAGGTTCTGGTTTTGTTACCTGTGTTGT
TGTTGGGATCATTAGCATTTCGTTGCATAAGAGACGTGTGAGGATTGAGAGAGCTGCGTT
TATAAGAGACATTTGCTTCTTCTGTGCTGCTATTGGTTCTTTGGCTTTGATTTTGGTCTA
TGGAAAGATCAATTTCTGGGGTGCTCTTGGGTTTTGTTCATTGTATGCTGTTTATGTTGC
CTTTGTGGTTCTCTCTGGGAGATT
   
Sequence Name 84-K033555-022-759-C08C-HP48
GenBank Accession LN514377 [GenBank]
Sequencing primer gene specific primer, HP48: 5'-AAATCTCCAAGAGAGAACCACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K033555-022-759-C08C-HP48
TGACAAACCCAGAGCACCCCAGAAATTGATCTTTCCATAGACCAAAATCAAAGCCAAAGA
ACCAATAGCAGCACAGAAGAAGCAAATGTCTCTTATAAACGCAGCTCTCTCAATCCTCAC
ACGTCTCTTATGCAACGAAATGCTAATGATCCCAACAACAACACAGGTAACAAAACCAGA
ACCTCCCACAACAGTGTTGAGACCAACATCATAAGTACCTTTAGATTCTCCCATGAAAGA
GACCAAACTTGCAAACAAATCAGGAGCACCATTACCAAGCGACAAAAGAGTAACACCAGC
AACAGTTGGTGACAAATTAAGCAGCTTTGAGAGACTTTCAAGAGATGAACAAAAGTACTC
AGAGGCCGTATGACCCAATAGATAGAACAAGAGAAGCAACCAGAGGAAGAGAAGAAACTA
CATGGATCAATATGAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11