Sequences from confirmation amplicon


Confirmation sequences for line 759F03 and hit At1g31280 are shown.
Line ID 759F03
Locus At1g31280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11182449
Primers used for PCR HM29 - 8474, show primer details
   
Sequence Name 08-K032516-022-759-F03C-8409
GenBank Accession LN489624 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K032516-022-759-F03C-8409
GAAGTCGCAACTTGCACCGATGGCTTCAGATTCATAACTTCAGCTACTGTCACATTTCAA
AAATATAATTACAACTTTGAGCTAAACAATAATCACATAAAACATTCATCAAAAGCTATT
GATTAACTAACATAAATCTCTCTAATGATTTCATAATTTAAGTTCTAATCATTTATTATA
AGCTTCTATGAATTTGCCCTAATTTCATTTCCCAAAGAGTTCTCGAACAAAATTAAAATT
GAGCATATATACCACGAACGGGTTCAGAAACCACGGTGGTGCTGGTGGACGGAGAAGCCG
AATCAGACGGAACCTGTGGCTTACGACCCCACGCGCCTCTACCAGCGACTCCTCCTAC
   
Sequence Name 08-K032516-022-759-F03C-HM29
GenBank Accession LN489625 [GenBank]
Sequencing primer gene specific primer, HM29: 5'-AGTAGGAGGAGTCGCTGGTAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K032516-022-759-F03C-HM29
GGCTTCTCCGTCACCAGCACCACCGTGGNTTTCTGAACCCGTTCGTGGTATATATGCTCA
ATTTTAATTTTGTTCGAGAACTCTTTGGGAAATGAAATTAGGGCAAATTCATAGAAGCTT
ATAATAAATGATTAGAACTTAAATTATGAAATCATTAGAGAGATTTATGTTAGTTAATCA
ATAGCTTTTGATGAATGTTTTATGTGATTATTGTTTAGCTCAAAGTTGTAATTATATTTT
TGAAATGTGACAGTAGCTGAAGTTATGAATCTGAAGCCATCGGTGCAAGTTGCGACTTCT
TATGTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
Primers used for PCR FQ35 - 3144, show primer details
   
Sequence Name 03-K033039-022-759-F03C-FQ35
GenBank Accession LN487375 [GenBank]
Sequencing primer gene specific primer, FQ35: 5'-CTGAAAATGTTCTTCTGACCATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K033039-022-759-F03C-FQ35
CGGGATTGTCAGTGAACCCTTGTCCCTAACCATAGCTAGCTCAAACCTCGAAACCTTCTT
GGTAGGAATTTCTCCTTTGATTTCAACATCATAATGTCTTATAACACTTTCAGGATTGAA
ATTCACTTTATAATGATTCACATATAGATTAACACGCCGCACAGCCACAACTCCGCCTCT
ATCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCT
GTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGG
GATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGAC
GTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGG
   
Sequence Name 35-K033039-022-759-F03C-3144
GenBank Accession LN501726 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K033039-022-759-F03C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAT
AGAGGCGGAGTTGTGGCTGTGCGGCGTGTTAATCTATATGTGAATCATTATAAAGTGAAT
TTCAATCCTGAAAGTGTTATAAGACATTATGATGTTGAAATCAAAGGAGAAATTCCTACC
AAGAAGGTTTCGAGGTTTGAGCTAGCTATGGTTAGGGACAAGGTGTTCACTGACAATCCC
GATGAGTTTCCCTTAGCTATGACAGCTTATGATGGTCAGAAGACNATTTTCA

Last Updated on 06.12.2018 16:11