Sequences from confirmation amplicon


Confirmation sequences for line 760B09 and hit At2g17800 are shown.
Line ID 760B09
Locus At2g17800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7741707
Primers used for PCR 34st - 8474, show primer details
   
Sequence Name 24-K116707-0022-760-B09C-34st
GenBank Accession LN497151 [GenBank]
Sequencing primer gene specific primer, 34st: 5'-AACTAGGCCTGGATTTTGGAGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K116707-0022-760-B09C-34st
GTTTCGTCATCATTTTTAATGAATTCCGCGATACGTTATGGATCATATCTCACATTTTCT
CTTGATAGTTAACCTCCATTGTAAGAGACAACTACATGCATGTAAGCAGCTGTTGGATGT
CTAGCAATTATAAACCTAATAGAAAAGGCTTATACAAGATGTTGTGCCAAAATTGGTGTA
ACGGATTCTGTTTCTTACTTTTAATACCAATGCTTAACTTTCGGCCTGCTGTCTGATCCG
TCTAGACCTACTTTTAATAGTTATGAGTTTCGTCTGGTGCTTATGTTTTGTGTTTCATCA
TATATGCCAGATCTTCGGGATGACAAACAGTTCTTCATTGACCACCCTGGCGCTGTACCA
ATTACTACTGCTCAGGTAAACAGAATTTTTCTTTGAAATGTGTCAATTTACTTAGCCTGT
TACTTAAACGAACCCTTTTAACCGTTCACCACTTTGGGCATCAAAGGGAGAGGAACTGAA
GAAACTAATGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGT
   
Sequence Name 11-K116706-0022-760-B09C-8409
GenBank Accession LN491288 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K116706-0022-760-B09C-8409
CCATTTACATTGAATATATCCATTAGTTTCTTCAGTTCCTCTCCCTTTGATGCCCAAAGT
GGTGAACGGTTAAAAGGGTTCGTTTAAGTAACAGGCTAAGTAAATTGACACATTTCAAAG
AAAAATTCTGTTTACCTGAGCAGTAGTAATTGGTACAGCGCCAGGGTGGTCAATGAAGAA
CTGTTTGTCATCCCGAAGATCTGGCATATATGATGAAACACAAAACATAAGCACCAGACG
AAACTCATAACTATTAAAAGTAGGTCTAGACGGATCAGACAGCAGGCCGAAAGTTAAGCA
TTGGTATTAAAAGTAAGAAACAGAATCCGTTACACCAATTTTGGCACAACATCTTGTATA
AGCCTTTTCTATTAGGTTTATAATTGCTAGACATCCAACAGCTGCTTACATGCATGTAGT
TGTCTCTTACAATGGAGGTTAACTATCAAGAGAAAATGTGAGATATGATCCATAACGTAT
CGCGGAATTCATTAAAAATGATGACGAAACATCACAGACAAGCTTGTAAACCTAGATTAT
TTCAGGATTTGTGATACTCCAAAA

Last Updated on 06.12.2018 16:11