Sequences from confirmation amplicon


Confirmation sequences for line 760D07 and hit At4g03600 are shown.
Line ID 760D07
Locus At4g03600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1604308
Primers used for PCR 87lx - 8474, show primer details
   
Sequence Name 60-K114067-0022-760-D07C-87lx
GenBank Accession LN510317 [GenBank]
Sequencing primer gene specific primer, 87lx: 5'-GATAATCTAGATCAAAATTCCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K114067-0022-760-D07C-87lx
AGTCAAATCTCTTCTAAAAGCAGAAAAAAGCTAAAACAGCACAAAAAGCAGCAACAACAT
TGCCTTCTTTCAAAAGAACCTTAAACACAAATCCGACAACTTCTCTCGTAATCTTCCTTC
CTTCAACCACTAAAACTCTTCTTGATTTCCCAAAGAAGGCAAGCAACACCACCACCGATA
TCCAAATCACCATCACCGCCGGAACCGCCACCGTCATAGCAGCCACCATCGACATAGCTC
CAAACATCGCACCGAGCACCACCGACGCGTATACCGCCGGTAATCTTGAAGGAGAATAAT
CTGAATTTGATGACGTGTATTGAAGCTTGTACCAGTTAAGAACTGATCTCAGGAGGTTCC
ATGAAGAAGATACGAGAATTGCGTAGACNGCGATGAAGAGACAAACCCATGTCCGTCTTT
TACTCATGATTGTGATGAAGATATGATACGATCTTGGAGATTCTGTTTGATCTGCCATGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 35-K114066-0022-760-D07C-8409
GenBank Accession LN501912 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K114066-0022-760-D07C-8409
GCCATTTCATGGCAGATCAAACAGAATCTCCAAGATCGTATCATATCTTCATCACAATCA
TGAGTAAAAGACGGACATGGGTTTGTCTCTTCATCGCTGTCTACGCAATTCTCGTATCTT
CTTCATGGAACCTCCTGAGATCAGTTCTTAACTGGTACAAGCTTCAATACACGTCATCAA
ATTCAGATTATTCTCCTTCAAGATTACCGGCGGTATACGCGTCGGTGGTGCTCGGTGCGA
TGTTTGGAGCTATGTCGATGGTGGCTGCTATGACGGTGGCGGTTCCGGCGGTGATGGTGA
TTTGGATATCGGTGGTGGTGTTGCTTGCCTTCTTTGGGAAATCAAGAAGAGTTTTAGTGG
TTGAAGGAAGGAAGATTACGAGAGAAGTTGTCGGATTTGTGTTTAAGGTTCTTTTGAAAG
AAGGCAATGTTGTTGCTGCTTTTTGTGCTGTTTTAGCTTATTTTCTGCTTATTAGAAGAG
ATTTTGACTCATATTAAGGTTCTTGATTTTTAGATTATTTAGGGAATTTT

Last Updated on 06.12.2018 16:11