Sequences from confirmation amplicon


Confirmation sequences for line 760D08 and hit At4g12650 are shown.
Line ID 760D08
Locus At4g12650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7470075
Primers used for PCR 24lu - 8474, show primer details
   
Sequence Name 08-K114086-0022-760-D08C-24lu
GenBank Accession LN489816 [GenBank]
Sequencing primer gene specific primer, 24lu: 5'-TTTTCTTGATTTTCGTTATTCGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K114086-0022-760-D08C-24lu
GCACAAGCAAGCAGTGAGAGAGATTATCAGCGTGACCTTCGAAACAACCCAAAGTCCCTC
GACCACCTCTTCTAGATCTGACTCCCTCGTAGATCTCCATACCTGGATCTCTCTCTCTCA
GGTTTGCTCTCGAATACTCTGAGATCTGTTGGATTCGCATCTGCTTCTTCTTTCGCGTTT
TCTGATTTTGTATTCATCGTTTTCGTATTCCATTATATCAATTAGCTTCTGAGTTTTGCG
TTTATGATCCATTTGAGATCTGATCTATTGATCCACCGATCTGATTCATTAATCCCGAAT
CTGGTTACTACGAATCTGATCTACATGCATAAACCATTTCGATTTCAATGTATTCGTGAG
AATGTGCTTGAATCTGTTACAATAGTAGTGATTTTTACTAATCGTGAGAGAGTTTTTGTA
TTTTGATACAGGAAGGGACAATGTTTGGTGTGTATCGAGTATTCGTGTTACTGGTGTTTG
TTAGTCAGCTTTGCAATGGGTTTTACTTACCAGGGAGTTATATGCACACATCTACATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 32-K114085-0022-760-D08C-8409
GenBank Accession LN500670 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K114085-0022-760-D08C-8409
GTAGATGTGTGCATATACTCCCTGGTAAGTAAAACCCATTGCAAAGCTGACTAACAAACA
CCAGTAACACGAATACTCGATACACACCAAACATTGTCCCTTCCTGTATCAAAATACAAA
AACTCTCTCACGATTAGTAAAAATCACTACTATTGTAACAGATTCAAGCACATTCTCACG
AATACATTGAAATCGAAATGGTTTATGCATGTAGATCAGATTCGTAGTAACCAGATTCGG
GATTAATGAATCAGATCGGTGGATCAATAGATCAGATCTCAAATGGATCATAAACGCAAA
ACTCAGAAGCTAATTGATATAATGGAATACGAAAACGATGAATACAAAATCAGAAAACGC
GAAAGAAGAAGCAGATGCGAATCCAACAGATCTCAGAGTATTCGAGAGCAAACCTGAGAG
AGAGAGATCCAGGTATGGAGATCTACGAGGGAGTCAGATCTAGAAGAGGTGGTCGAGGGA
CTTTGGGTTGTTTCGAAGGTCACGCTGATAATCTCTCTCACTGCTTTGCTTTGGTTGCTT
AATGCGTTCGCAAAGAGAGCGAATAACGAAACCAAAGAAAAACGGTGNGNGAGATTATCA
GCGTGACCTTCGAAACACCCAAAGTCCCTCGACCACCTCTTCTAGATCTGACTCACTCGT
AGATCTCCATACCTGGATCTCTCTCTCTCAGGTTTGCTCTCGAATACTCTGAGATCTGTT
GGATTCGCATCTGCTTCTTCTTTCGCGTTTTCTGATTTTGTATTCATCGTTTTCGTATTC
CATTATATCAATTAGCTTCTGAGTTTTGCGTTNATGATCCATTTGAGATCTGATCTATTG
ATC

Last Updated on 06.12.2018 16:11