Sequences from confirmation amplicon


Confirmation sequences for line 760D09 and hit At1g14170 are shown.
Line ID 760D09
Locus At1g14170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4843506
Primers used for PCR NL75 - 8474, show primer details
   
Sequence Name 70-K103789-0022-760-D09C-NL75
GenBank Accession LN512377 [GenBank]
Sequencing primer gene specific primer, NL75: 5'-CCTTTGTGAAAGTGAAACTAAGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K103789-0022-760-D09C-NL75
GAAGTCCTGATGCCGCTATGAAAGCTCTCACGCAAGTAATCTTGCGATTAAGAGCTAACT
CTTTCGACATGGATCATGGACTTGTCTTGCTACCAACATCTTTTCCATATATCCCTCAAG
TGACTGAAAGTTCGAGCAAATCCAAATACGCAAAACGCGATGACCACAGTAAACTGGTAT
GTACTGGTCCACAAGATTTTTTTAAAAAATATCAAATGTAATATGCATCAATCAATCAAA
GATATTCACTTAAGTAATTTATATGCTAAGAGAAAGAAACCATGTTTCTTAAAAGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
TACACAACGTTATA
   
Sequence Name 78-K103790-0022-760-D09C-8409
GenBank Accession LN513679 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K103790-0022-760-D09C-8409
GCATTTACATTGAATATATCTTTTAAGAAACATGGTTTCTTTCTCTTAGCATATAAATTA
CTTAAGTGAATATCTTTGATTGATTGATGCATATTACATTTGATATTTTTTAAAAAAATC
TTGTGGACCAGTACATACCAGTTTACTGTGGTCATCGCGTTTTGCGTATTTGGATTTGCT
CGAACTTTCAGTCACTTGAGGGATATATGGAAAAGATGTTGGTAGCAAGACAAGTCCATG
ATCCATGTCGAAAGAGTTAGCTCTTAATCGCAAGATTACTTGCGTGAGAGCTTTCATAGC
GGCATCAGGACTTCCTGTAATCTAACCACAGCAATATAACAGGAAACATTGGCTTAGTTT
CACTTTCACAAAGG

Last Updated on 06.12.2018 16:11