Sequences from confirmation amplicon


Confirmation sequences for line 760E02 and hit At1g63830 are shown.
Line ID 760E02
Locus At1g63830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23687052
Primers used for PCR 01di - 8474, show primer details
   
Sequence Name 52-K109780-0022-760-E02C-01di
GenBank Accession LN507846 [GenBank]
Sequencing primer gene specific primer, 01di: 5'-CTCAGATACTCTCTTGCTGTGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K109780-0022-760-E02C-01di
TTCTGTGATTTTAGATTCTCCCTCTAATCTATTCATGTTCACGAATTGCTAATATTTTGC
GGTCTTGATTTTCTTGCAGGGTCTGCGCATGTATGCAGGTACTAGAAAGCGAGAATTAGA
AACTTATCTGAGCTTCTGAAACTATCTTCTCTTATGGTATATATATATAACCCAAAATCC
AATTTTCTGCTTGCAGACACAACACAAACTTGAGATGGACAAAAGAGATGGAGTGTTTGG
ATCGCAGCCAATGGGTGTGCCACCAGCTCAGCAGATGTCTCGTTTTGATCAACCTGTCCC
TCCTCCAGTCGGATACCCTCAGTCCTATCCACCGCCTGCTCAAGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 36-K109779-0022-760-E02C-8409
GenBank Accession LN502259 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K109779-0022-760-E02C-8409
GCCATTTACAATTGAATATATCCTTGAGCAGGCGGTGGATAGGACTGAGGGTATCCGACT
GGAGGAGGGACAGGTTGATCAAAACGAGACATCTGCTGAGCTGGTGGCACACCCATTGGC
TGCGATCCAAACACTCCATCTCTTTTGTCCATCTCAAGTTTGTGTTGTGTCTGCAAGCAG
AAAATTGGATTTTGGGTTATATATATATACCATAAGAGAAGATAGTTTCAGAAGCTCAGA
TAAGTTTCTAATTCTCGCTTTCTAGTACCTGCATACATGCGCAGACCCTGCAAGAAAATC
AAGACCGCAAAATATTAGCAATTCGTGAACATGAATAGATTAGAGGGAGAATCTAAAAAT
CACAAGAAGTAAATACAGACGTGCAGTAGACCATATCAGCACAGCAAGAGAGTATCTGAG

Last Updated on 06.12.2018 16:11