Sequences from confirmation amplicon


Confirmation sequences for line 760F03 and hit At3g52670 are shown.
Line ID 760F03
Locus At3g52670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19525978
Primers used for PCR CR92 - 8474, show primer details
   
Sequence Name 55-K107248-0022-760-F03C-CR92
GenBank Accession LN508830 [GenBank]
Sequencing primer gene specific primer, CR92: 5'-GATTGTTTGCAAGTCTTTCCTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K107248-0022-760-F03C-CR92
GAGTTTGCATCTCGTTTAGATGAAGTCAGTTAATCCTGTAGATATCGGACTTTGGATAGG
AATTGCGTTTGCTCGACATTTGCGTGAATTGGTACTCTATGTTGCCCCGAAGCAGACCTT
CACATTTCCGAGTAGCTTGTGTATTTGTAACACACTTGAGACTTTGAAACTCATACTTGG
GATTCATGTAGACATACCTTGTCCGGTTCTTTTGAAATCTCTTAGAACTCTGCACCTTGA
TTCTGTGAGCTACAAAGATGAAGAATCTATCCGTAACTTGTTATCTAGTTGCCCTATTCT
TGAAAATTTGGTTGTGTATGAATATTGGTATAATGTTGTGAATTTCGATATTGAGGTCCC
GTCTTTGAAGAGACTTGAGATTTGTGACGTTCTTCACAAAAAAGAGTTTAGAAGATATAC
GATTAATGTTCCCTGTTTGAAATACTTGAGAATCGAAGGGTTAAATAAAGATTTTGAGCT
TACCAATAATAAAGAAACCATTATTATCATGACATTATTGCTGATCCAAGTACTTACCAA
TTTTAAATGTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 39-K107247-0022-760-F03C-8409
GenBank Accession LN503374 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K107247-0022-760-F03C-8409
GAGCCATTTACAATTGAATATATCCTGTACATTTAAAATTGGTAAGTACTTGGATCAGCA
ATAATGTCATGATAATAATGGTTTCTTTATTATTGGTAAGCTCAAAATCTTTATTTAACC
CTTCGATTCTCAAGTATTTCAAACAGGGAACATTAATCGTATATCTTCTAAACTCTTTTT
TGTGAAGAACGTCACAAATCTCAAGTCTCTTCAAAGACGGGACCTCAATATCGAAATTCA
CAACATTATACCAATATTCATACACAACCAAATTTTCAAGAATAGGGCAACTAGATAACA
AGTTACGGATAGATTCTTCATCTTTGTAGCTCACAGAATCAAGGTGCAGAGTTCTAAGAG
ATTTCAAAAGAACCGGACAAGGTATGTCTACATGAATCCCAAGTATGAGTTTCAAAGTCT
CAAGTGTGTTACAAATACACAAGCTACTCGGAAATGTGAAGGTCTGCTTCGGGGCAACAT
AGAGTACCAATTCACGCAAATGTCGAGCAAACGCAATTCCTATCCAAAGTCCGATATCTA
CAGGATTAACTGACTTTCATCTAAAACTGAGATGCAAACTCTTTAGAACCGGAGCTTTGA
GTGAAAGGAAAGACTTGCAAACAAT

Last Updated on 06.12.2018 16:11