Sequences from confirmation amplicon


Confirmation sequences for line 760G03 and hit At4g03420 are shown.
Line ID 760G03
Locus At4g03420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1513247
Primers used for PCR CR94 - 8474, show primer details
   
Sequence Name 21-K107428-0022-760-G03C-CR94
GenBank Accession LN495688 [GenBank]
Sequencing primer gene specific primer, CR94: 5'-GCAGCTTCATAAGATCAGCTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K107428-0022-760-G03C-CR94
TAGGCGAAAAGTATGTATGTATAGATTAGAATATATTATTATTCAAGACTTGAAATGAGA
CATATGATTTTGGTTGATTTCAGATCAATGAATTGGCTCAAAGGTACCCGGGACTAATGT
CGTTGCGAAGCGTAGATCTTTCCCCGGCAAGTTGGATGGCGGTAGCGTGGTACCCTATTT
ACCATATTCCAATGGGAAGGACCATCAAAGATTTGTCCACTTGTTTCCTCACTTATCACA
CTCTTTCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAT
   
Sequence Name 05-K107427-0022-760-G03C-8409
GenBank Accession LN488381 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K107427-0022-760-G03C-8409
TCATTGAATATGAAAGAGTGTGATAAGTGAGGAAACAAGTGGACCAATCTTTGATGGTCC
TTCCCATTGGAATATGGTAAATAGGGTACCACGCTACCGCCATCCAACTTGCCGGGGAAA
GATCTACGCTTCGCAACGACATTAGTCCCGGGTACCTTTGAGCCAATTCATTGATCTGAA
ATCAACCAAAATCATATGTCTCATTTCAAGTCTTGAATAATAATATATTCTAATCTATAC
ATACATACTTTTTCGCCTATTGCTAATTAGGAATCAACCAAGTGACTAATCCTAGTAATC
AAAGAAGCTGATCTTATGAAGCTG

Last Updated on 06.12.2018 16:11