Sequences from confirmation amplicon


Confirmation sequences for line 761F11 and hit At1g72110 are shown.
Line ID 761F11
Locus At1g72110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27131461
Primers used for PCR NL77 - 8474, show primer details
   
Sequence Name 42-K104195-0022-761-F11C-NL77
GenBank Accession LN504447 [GenBank]
Sequencing primer gene specific primer, NL77: 5'-CTTACATCTGAATGAGAGGAACCC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K104195-0022-761-F11C-NL77
TTTGATATTGATTTTGAAATGGAAAATCATGAAACTTAACTTATAAATTAACAATAAAAA
TGCGTGTACATGTGCATATTTGTATATGTGTGTGTACGTTGATTACGTACCAGTATGCTA
GAGAAGCGTGGATGGTTGATCAATGTGTTCTTTATGCCTTCGACGATGGTTGAGGCATTG
CCTTCGGTTTTGCATCCAATGGTTACAATGTTGAAGACATCGAGACCCGGCAAGCTAAAC
AAGCGTGCAAATGGACTTACTGGCTCCTCTGCTTCCGTCACTTGCCTTTCTATTGCCATA
TCTCTCTCTCTTTCCAATTTCTAGTTTTATGGTATTATTTTACAAATTACATATATATGC
AAATGGTTTATTTATAAACACATAATAGCTATTAATTAAATGAGGTTGTTGTTAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTAT
   
Sequence Name 58-K104196-0022-761-F11C-8409
GenBank Accession LN509693 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K104196-0022-761-F11C-8409
CCTTTCAATTGAATTATCCTAACAGCACCTCATTTCATTTATATCTATTATGTGTTTATA
AACAAACCATTTGCATATATATGTAATTTGTAACATAATACCATAAAACTACAAATTGGA
AAGAGAGAGAGATATGGCAATAGAAAGGCAAGTGACGGAAGCAGAGGAGCCAGTACGTCC
ATTTGCACGCTTGTTTAGCTTGCCGGGTCTCGATGTCTTCAACATTGTAACCATTGGATG
CAAAACCGAAGGCAATGCCTCAACCATCGTCGAAGGCATAAAGAACACATTGATCAACCA
TCCACGCTTCTCTAGCATACTGGTACGTAATCAACGTACACACACATATACAAATATGCA
CATGTACACGCATTTTTATTGTTAATTCATAAGTTAAGTTTCATGATTTTCCATTTCAAA
ATCAATATCAAATGAGTGTATTGATCCATATCCACAATATATTACTTTAGGGTTCCTCTC
ATTCAGATGTAA
Primers used for PCR NL76 - 8474, show primer details
   
Sequence Name 34-K118262-0022-761-F11C-NL76
GenBank Accession LN501588 [GenBank]
Sequencing primer gene specific primer, NL76: 5'-AGAGTTTTACAGAGCCCAATACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K118262-0022-761-F11C-NL76
ATTGAGGGGGAACCAGAATCTAGGTGAATATTAAACCTAATGTCACTCTTTGATAGACTA
ATGTCTTTCTTTTGAAACAATGAGAATTAATAACTAGTACTCTAGTAGTACCCTTCACCA
ACAACAAGCACGGACGTATGGCCTTCCTTTTCATTTATTATACACTGAAGAGTTCTTGCC
ACAGAAATAGAAATGTTAAGTTCAATTTCGAAATACGTGGATACTTTTCTGACACACCTT
TTTTTTTTTTGGCCCCCCCTTTCCCCAGGAATTTTTTCCTTTGAAATTTTGGTTAAAAAA
AAATTTTTTAAGAATCCCCCTCCCCCCCTTTTCCAAAAATTGAAAATGGCAAAACCCCCC
TTAAAGGGGGGCAATTAAAATTTTTCCAAAAATCCCCCCTTTTTTTGGGACCTTTTGAAA
ATTTTTTTTGGGGGGAAAAAAAAAATTTTTTACCTTCTTGGAAAAAAACCCTTTAAAAAC
CCCCATTGGAAAGGGGTTCCTTCCCGGGAAATTTTCCGGGATCCCCCATGAATTTAAGGG
GCAATTTGGGAAAAAAAAAAAAATTTTTTCCCTTTTTTTTTTCTTTCCAAAATTTAAA
   
Sequence Name 14-K118261-0022-761-F11C-8409
GenBank Accession LN492710 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K118261-0022-761-F11C-8409
TTACATTGAGGTTATTAAATGGTTTATTTACAAGAAGCTAACTAACTCTTCTTTACCACA
CTATATATATGTACGATAGGTCCAAGAGATAGTGGCGATTGTCGAGAGAATTATAATTGA
CACCATTCATAGGGGTTTTATGCATTCTCTATCTCTTGTAATTGTCGGTACGTGGATTCA
TGAGATATTTTTCTTCAACCAAAATTTCAAGAGATAATACTCCTAGTGAAAGAGGATGAC
AAAAAAAAAAAAGGGGGGTCAAAAAAATTTCCCCCTTTTTCAAAATTGAACTTAAATTTT
TTTTTTTTGGGGGAAAAACCTTTCCGGGTTAAAAAAATGAAAAGGAAGGCCCAACCCCCC
GGCTTTTTTTTGGGGAAGGGTACCCCCAAGGTACCAGTTTTTAATTCCCCTTGTTTCAAA
AAAAAAAATTTTTTTTTTCAAAAAGGGACCTTGGGTTTAAATTTCCCCTAAATTCCGGAA
CCCCCCCAAGGGTTTGGGGAAAATCCCAAATTTGGGTTTAAAAAAGGGTTCAAAACCCAG
GGGTTTTGGG

Last Updated on 06.12.2018 16:11