Sequences from confirmation amplicon


Confirmation sequences for line 762A06 and hit At1g67940 are shown.
Line ID 762A06
Locus At1g67940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25478601
Primers used for PCR LB09 - 8474, show primer details
   
Sequence Name 66-K033720-022-762-A06C-8409
GenBank Accession LN511536 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K033720-022-762-A06C-8409
TTGAAAAGAACTTCAACAATCTCTCCGTCGACAACACGGCAAACGATATCAGCAACTTTC
TGAATCTGCTTGATACTGTGAGAAACAATCACAGTAGTAATCCCTCTCTGCTTCTTCAGT
TTCACTATAACATCCTCAATGTTCTCTGTCGATATCGGATCAAGAGCACTTGTTGGTTCA
TCGAGCAGCAACACCTCAGGCTCGTTGGCTAGAGTCCTTGCAAGTGCTACTCGTTGAGCT
TGACCCACAGATAACTCTGCACCAGTCTTCTTAGCAAAGGAAGCATCAAGGTCTGCAAGA
CTTAGCAGCTTATAAACCTCTTCGTCACTTAGTTTCTCCCCTCTCAAATTCGGACCATAT
CTCACATTATCCGCAACAGTCCCTGTAACCAACCACACATTATCAAGACCTATGAAACTA
GAGGCTAAGCAAATCCCAAAATTTGATTATTACCTTGAAAAAGAACAGGAAGCTGAAAAA
GCATTCCAACTCTACGA
   
Sequence Name 28-K033720-022-762-A06C-LB09
GenBank Accession LN498742 [GenBank]
Sequencing primer gene specific primer, LB09: 5'-GTCGTAGAGTTGGAATGCTTTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K033720-022-762-A06C-LB09
TATAATCAAATTTTGGGATTTGCTTAGCCTCTAGTTTCATAGGTCTTGATAATGTGTGGT
TGGTTACAGGGACTGTTGCGGATAATGTGAGATATGGTCCGAATTTGAGAGGGGAGAAAC
TAAGTGACGAAGAGGTTTATAAGCTGCTAAGTCTTGCAGACCTTGATGCTTCCTTTGCTA
AGAAGACTGGTGCAGAGTTATCTGTGGGTCAAGCTCAACGAGTAGCACTTGCAAGGACTC
TAGCCAACGAGCCTGAGGTGTTGCTGCTCGATGAACCAACAAGTGCTCTTGATCCGATAT
CGACAGAGAACATTGAGGATGTTATAGTGAAACTGAAGAAGCAGAGAGGGATTACTACTG
TGATTGTTTCTCACAGTATCAAGCAGATTCAGAAAGTTGCTGATATCGTTTGCCTTGTTG
TCGACGGAGAGATTGTTGAAGTTCTTATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11