Sequences from confirmation amplicon


Confirmation sequences for line 762B02 and hit At2g42120 are shown.
Line ID 762B02
Locus At2g42120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17564524
Primers used for PCR MC60 - 8474, show primer details
   
Sequence Name 93-K035353-022-762-B02C-MC60
GenBank Accession LN515227 [GenBank]
Sequencing primer gene specific primer, MC60: 5'-GAAGACGAGAGTGGGAGAGTTAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K035353-022-762-B02C-MC60
TGCGATTATGTGAAGGTATTGCAAATGGGTTCTTACTGTTTTTACTGTATGATTTTTTCC
TTCTTTACAATGTGGCAAATCTTAGAGATTTTGATCAAGCTTTCCTCTCTTAAAAGATGG
GTTCTTTAAGAAAATTAACGTTGAAGCCTCCCGTGCATTGTAGGTGTTGTTGTTGCACTG
CATGGGAAGGAAACTAATGCTGGTGAATTCTTTGTTGAGGATGTACTAGAAGCTGGTTTA
CCACCTCAGATTGAGCGGCCTATCGATCTACGTAAGTCTAGCTATGTTCTCTTCCTTTTG
CTAACCTCATGGCTCAATCATTTCTATAAGCAATCTCTCATGATACATCCATATTGCATC
TGCAGAGGAAGATAAATATGTCGTGTTATTGTCGGGCCTTTGTATTGGAAGCAAATCGGC
TAATCCCCTGCAGTTTCAGCTTCTTGTTGACCATATAACTGGGCATCTCGGAGATGAGGA
GGTTCAAATCTCTTAACTTGCAGGTTGTTCAACATATTTCTTTCCTTAATTTATACTTTA
TGGTTTGAACAGGAACAAGGCCTTGCAGCACAGATAGTTCATGTAGTANTTGCTGGAAAC
TC
   
Sequence Name 45-K035353-022-762-B02C-8409
GenBank Accession LN505445 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K035353-022-762-B02C-8409
GAGCCATTTAAATTGAATATATCCTGGCCATTAATGAGTTTGCGGGGATATTCAAAAGAG
TTTCCAGCAATTACTACATGAACTATCTGTGCTGCAAGGCCTTGTTCCTGTTCAAACCAT
AAAGTATAAATTAAGGAAAGAAATATGTTGAACAACCTGCAAGTTAAGAGATTTGAACCT
CCTCATCTCCGAGATGCCCAGTTATATGGTCAACAAGAAGCTGAAACTGCAGGGGATTAG
CCGATTTGCTTCCAATACAAAGGCCCGACAATAACACGACATATTTATCTTCCTCTGCAG
ATGCAATATGGATGTATCATGAGAGATTGCTTATAGAAATGATTGAGCCATGAGGTTAGC
AAAAGGAAGAGAACATAGCTAGACTTACGTAGATCGATAGGCCGCTCAATCTGAGGTGGT
AAACCAGCTTCTAGTACATCCTCAACAAAGAATTCACCAGCATTAGTTTCCTTCCCATGC
AGTGCAACAACAACACCTACAATGCACGGGAGGCTTCAACGTTAATTTTCTTAAAGAACC
CATCTTTTAAGAGAGGAAAGCTTGATCAAAATCTCTAAGATTTGCCACATTGTAAAGAAG
GAAAAAATCATACAGTAAAAACAGTAAGAACCCATTTGCAATACCTGTCACATAAATCGC
AGGTGAAAGTGCGGAACCAGCAAGCTTAACTCTCC

Last Updated on 06.12.2018 16:11