Sequences from confirmation amplicon


Confirmation sequences for line 762E02 and hit At4g04900 are shown.
Line ID 762E02
Locus At4g04900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2486895
Primers used for PCR FQ63 - 8474, show primer details
   
Sequence Name 10-K033884-022-762-E02C-FQ63
GenBank Accession LN490554 [GenBank]
Sequencing primer gene specific primer, FQ63: 5'-TGAAATAGAAATTGGGTTTCCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K033884-022-762-E02C-FQ63
GGCTGGGAGGGTTCATCTGGTAGTGCTCCTGGTTGGGTAAGCTCTTTAGTGCCTTTTCGG
CCTATATGTGAAAATTCTAGTTTCTAACAAGTAAAACCTAAAAAAGAAGTAAAATTCATA
TAGCTAATCAGTGAATATTTGTATTTCTTGTGAAGATGAGTGAGTTTAAGGTCGGGGCTG
AGCTTTTATCTCCAAGACCATCATCTTTCAGTAATGCAAGACCTTCTACTTCTTTCTTCA
CTTCTTCTTCTTCCACTGGTAAGTTATTTATTTGTTTTTAACTTTTTCATTTCATTGGTT
TTTTTTCTTAATCGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 50-K033884-022-762-E02C-8409
GenBank Accession LN507081 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K033884-022-762-E02C-8409
TGAAGCCATTTACAATNNTGAATATATCCTGACGATTAAGAAAAAAAACCAATGAAATGA
AAAAGTTAAAAACAAATAAATAACTTACCAGTGGAAGAAGAAGAAGTGAAGAAAGAAGTA
GAAGGTCTTGCATTACTGAAAGATGATGGTCTTGGAGATAAAAGCTCAGCCCCGACCTTA
AACTCACTCATCTTCACAAGAAATACAAATATTCACTGATTAGCTATATGAATTTTACTT
CTTTTTTAGGTTTTACTTGTTAGAAACTAGAATTTTCACATATAGGCCGAAAAGGCACTA
AAGAGCTTACCCAACCAGGAGCACTACCAGATGAACCCTCCCAGCCAATATGAGCCACAT
GTTTTACATCTGTTGGAAACCCATTTTCTATTTCAATGGAGAATTAAAGAGGCCATATTT
ACCAAATTC

Last Updated on 06.12.2018 16:11