Sequences from confirmation amplicon


Confirmation sequences for line 763B01 and hit At5g48770 are shown.
Line ID 763B01
Locus At5g48770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19774710
Primers used for PCR 17di - 8474, show primer details
   
Sequence Name 69-K109782-0022-763-B01C-17di
GenBank Accession LN512226 [GenBank]
Sequencing primer gene specific primer, 17di: 5'-TGTATGATATTCAGTCGCTCTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K109782-0022-763-B01C-17di
GAATCTGACCAACTTCCGATCTTTCAGGGCAACAAAGCTTGAGGACCTGATTGCTAAAGG
TTGCACGAGGTTGGAGCAAATCCCGGAAACAATTGGTAGCTTACCTTCACTGAAGAAACT
TGATGTATCCCACTGTGATCGCCTCATCAATCTTCAGATGATTATAGGAGAATTGCCTGC
CCTTCAAAAACGTAGCCCAGGGCTTTTTCGGCAAGCTTCATTGAGTTTCCCAGATGCTGT
AGTTACACTGAACTCTCTTACGAGTCTAGCCATCCATGGCAAATTAAATTTTTGGTTGTC
ACATCTCCGAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
   
Sequence Name 66-K109781-0022-763-B01C-8409
GenBank Accession LN511632 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K109781-0022-763-B01C-8409
ATTTACAATTGAATATATCCTCGGAGATGTGACAACCAAAAATTTAATTCGCCATGGATG
GCTAGACTCGTAAGAGAGTTCAGTGTAACTACAGCATCTGGGAAACTCAATGAAGCTTGC
CGAAAAAGCCCTGGGCTACGTTTTTGAAGGGCAGGCAATTCTCCTATAATCATCTGAAGA
TTGATGAGGCGATCACAGTGGGATACATCAAGTTTCTTCAGTGAAGGTAAGCTACCAATT
GTTTCCGGGATTTGCTCCAACCTCGTGCAACCTTTAGCAATCAGCTCCTCAAGCTTTGTT
GCCCTTGAAAGATCTGGAAGTTTGGTCAGATTCTTAGATCCAGTGACATCTAGCATCCTT
AACTCCTAGAGCGACTGAATATTATACA

Last Updated on 06.12.2018 16:11