Sequences from confirmation amplicon


Confirmation sequences for line 764A03 and hit At1g69870 are shown.
Line ID 764A03
Locus At1g69870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26316125
Primers used for PCR CN07 - 8474, show primer details
   
Sequence Name 36-K030758-022-764-A03C-8409
GenBank Accession LN502104 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K030758-022-764-A03C-8409
TGAGCCATTTACATTGAATATATCCTGCCGAAAAAGTGAGAATATATTCTCACATTATGG
AATCCACCCCGAAATCGAAGTGAGTATATCACGAGTGTAGTAATATCTTAGAGATGGTTT
TGGAGGATAGAAAGGACGGTTCTTCTTTGCCGGGACGATCCGGTAGTTTCTCTAAATCGT
CACCGTCGGAGTTGGATGTTGTTGATCCCTACAAGCGGATAAGTTCGCCGGGATCTATAT
TGGATGCTGAGAAGGTAGAGAAAAAGCCTGGAGGATGGAGAGCCGTCTCGTTCATTTTAG
GTATTTTAATTTTATTTTTAGGCTTCGTGTACATAATTTAGTTTACCTAATCCTTGACCT
TTCCTCAGTTATTGGCTAATATTTTTTTGTTAATTTCCTTAATTGGATATATAGTTCTAG
AAACCAGTTAATATATTATCATTGGGAGATGTAACGGGCCGTTGCAAGATCACGTTCTGC
CTAAATCATTGCACATAATAGTCAGAACAAAAGAAATGAGCGCATAAT
   
Sequence Name 75-K030758-022-764-A03C-CN07
GenBank Accession LN513166 [GenBank]
Sequencing primer gene specific primer, CN07: 5'-AGATTATGCGCTCATTTCTTTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K030758-022-764-A03C-CN07
TGGGTTCTGGAACTATATATGCAATTAAGGAAATGAACAAAAAAATAGTGGGCAATAACT
AAGGAAAGGTCAAGGATTAGGTAAACTAAATTATGTACACGAAGCCTAAAAATAAAATTA
AAATACCTAAAATGAACGAGACGGCTCTCCATCCTCCAGGCTTTTTCTCTACCTTCTCAG
CATCCAATATAGATCCCGGCGAACTTATCCGCTTGTAGGGATCAACAACATCCAACTCCG
ACGGTGACGATTTAGAGAAACTACCGGATCGTCCCGGCAAAGAAGAACCGTCCTTTCTAT
CCTCCAAAACCATCTCTAAGATATTACTACACTCGTGATATACTCACTTCGATTTCGGGG
TGGATTTAATGTTGTGAGAATATATTCTCACTTTTTCGGCAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCAC
GTCCAAAT
Primers used for PCR CN06 - 8474, show primer details
   
Sequence Name 78-K030759-022-764-A03C-CN06
GenBank Accession LN513624 [GenBank]
Sequencing primer gene specific primer, CN06: 5'-TCACAATACGTGGAACAAAACAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K030759-022-764-A03C-CN06
ACAAAAAACATAGCACTAAAATAGTTTTCTTTTAATTTTCCTCTTAAACTCCTACGAGGA
GGCTACTAGTGGCGAACGTCATTCAAATTTACCCACACCCACCATATATTATCTACTCAC
TCCCTTATATATACACACTTCCACAAATAATTCACTACACCCAAAAAAGCTTCTAACAAA
GAGAAAGTGTCTGTATGTTGTGCCCACTCTCTACATTTATCAATCACTTCATGTGTGTTA
CCTTGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTACATTCACGTCCAAATGG
   
Sequence Name 39-K030759-022-764-A03C-8409
GenBank Accession LN503247 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K030759-022-764-A03C-8409
ATTTACAATTGAATATATCAAGGTAACACACATGAAGTGATTGATCCATGTAGAGAGTGG
GCACAACATACAGACACTTTCTCTTTGTTAGAAGCTTTTTTGGGTGTAGTGAATTATTTG
TGGAAGTGTGTATATATAAGGGAGTGAGTAGATAATATATGGTGGGTGTGGGTAAATTTG
AATGACGTTCGCCACTAGTAGCCTCCACGTGAGAAGTTTGAAGAGGAAAATTAAAAGAAA
ACTATTTTAGTGCTATGTTTTTTTTGTTTTTCTTACACCGTACGTAACATTATTGTTTTG
TTCCACGTATTGTG

Last Updated on 06.12.2018 16:11