Sequences from confirmation amplicon


Confirmation sequences for line 764B08 and hit At2g01670 are shown.
Line ID 764B08
Locus At2g01670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:296966
Primers used for PCR NL81 - 8474, show primer details
   
Sequence Name 72-K103929-0022-764-B08C-NL81
GenBank Accession LN512716 [GenBank]
Sequencing primer gene specific primer, NL81: 5'-AAGAGAATGTCTTGAAGAAGCTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K103929-0022-764-B08C-NL81
AATCTTTTTGTTGTTATGTTTTGTTTGATATTTGTTGTATTGTTTTGTTCTTAATTTTGT
TTGGTTTTGATTATTTCAGCACCAACTTGGAAAATGGGATTTTTTAAGCAAAAGCAGAGG
AACATACTATGAAGGACTAATGTTCCCTATGCTTGTTACAGAACAACTTGAGCTTTGGCC
TGAACAACATGTTAGGCAAAGAATCTGGGTCAGTAGCTAAATAAAACTATGTTTTCTTTA
GATATTTTGTAACATATCTAGTGTTTTACTGATTGATATGAATTTATTGGGGACAACAGA
TGAATGTAACC
   
Sequence Name 88-K103930-0022-764-B08C-8409
GenBank Accession LN514861 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K103930-0022-764-B08C-8409
CCAAAACATCTAATGCTTCTTTCATCCACCAATCTCTACAAGCTTCTCTTGCTTCGGTTA
CATTCATCTGTTGTCCCCAATAAATTCATATCAATCAGTAAAACACTAGATATGTTACAA
AATATCTAAAGAAAACATAGTTTTATTTAGCTACTGACCCAGATTCTTTGCCTAACATGT
TGTTCAGGCCAAAGCTCAAGTTGTTCTGTAACAAGCATAGGGAACATTAGTCCTTCATAG
TATGTTCCTCTGCTTTTGCTTAAAAAATCCCATTTTCCAAGTTGGTGCTGAAATAATCAA
AACCAAACAAAATTAAGAACAAAACAATACAACAAATATCAAACAAAACATAACAACAAA
AAGATTTTAACTTTAAAATACCTCAACGTTGCCAAGAACTCCAGCTTCTTCAAGACATTC
TCTT

Last Updated on 06.12.2018 16:11