Sequences from confirmation amplicon


Confirmation sequences for line 764F03 and hit At5g02780 are shown.
Line ID 764F03
Locus At5g02780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:631122
Primers used for PCR 07dc - 8474, show primer details
   
Sequence Name 26-K109029-0022-764-F03C-07dc
GenBank Accession LN497964 [GenBank]
Sequencing primer gene specific primer, 07dc: 5'-ATTAGATCAAGACTCTCCCCAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K109029-0022-764-F03C-07dc
AGGAAGATCTCTAGGAACCCATTTGATCTCATCTTGATGACCCTTAAACTCCCCAATGTC
AAACTAATCAACTAATCTTGCTTATAAACTTTTCATCTCGATCTCGATTTACATACATAT
ATACCATAAATATATAAAAGACAAGTACCTTGAGGTTTCTTGTAATCCAAACACGTTGTG
CAAATGGGCAAGTGTAGCTTATGTACAACCTTGTGGTTCCGTCGAACAGAGCAGGTGGAT
CAAAGGTAGCATCGAGCGGAACCTGACGATCTTCTACGAAAATTTTTCTTGATAAAGGAA
AAAAAATCAAGGAGATGAATATAGATTTATGTCCGGGAAATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATAAT
   
Sequence Name 10-K109028-0022-764-F03C-8409
GenBank Accession LN490695 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K109028-0022-764-F03C-8409
CCACTGATCCACCTGCTCTGTTCGACGGAACCACAAGGTTGTACATAAGCTACACTTGCC
CATTTGCACAACGTGTTTGGATTACAAGAAACCTCAAGGTACTTGTTTTTTATATATTTA
TGGTATATATGTATGTAAATTGAGATCGAGATGAAAAATTTATAAGCAAGATTTGTTGAT
TTGTTTGACATTGGGGAGTTTTAGGGTCTGCAAGATGAGATCAAATTGGTTCCTATAGAT
CTTCCTAATAGGCCAGCTTGGTTAAAGGAGAAAGTTAATCCTGCAAACAAGGTGCCGTTT
ATTTTTATGATTAATCCCTATAAACTAATTAACGTTCTTTCAAAAGTGACAATAGAAGAC
TCTAAAAGATTGTGATAATGATGCATGTAGGTTTCCTGCCCTTGAACACAATGGAAAAAT
CACTGGG

Last Updated on 06.12.2018 16:11