Sequences from confirmation amplicon


Confirmation sequences for line 764G06 and hit At5g02700 are shown.
Line ID 764G06
Locus At5g02700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:609483
Primers used for PCR BW33 - 8474, show primer details
   
Sequence Name 10-K104352-0022-764-G06C-BW33
GenBank Accession LN490636 [GenBank]
Sequencing primer gene specific primer, BW33: 5'-TGCTTAGTTTCAACAATTCAATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K104352-0022-764-G06C-BW33
TTTATATTATGAATGTGTATTAAAATTTTAGTCTAAAGGCTTGACTAGTAAGTATGTCAG
ATTAATAAGTGAATTATGAAATTACGTAGTCAATAGAGGGACAACAATTTTAAGCTAAAA
GATATTAATTTTGTTTAAAACCGAAAAAAAATATCTATTATTCTATTGATGAATGGAATT
TTTAAATTTTGGTTTAATTTTAAAACGTGCATGGTAAACTGAAAATTCACTTGGTACGTA
TTTTTTACGTACTATTATTTAACACAAGTTTTAAGAAAATGTATGTATTTAGCGGAATCA
CATTTTCAAAACGTAGAAAACTGCATAACATTCAAGGCTAAATAACACAATACAATGTAG
TATTTTTAGCAATTGCTTTGTTTGAGCAAAATGGAGACATTATTGTTGCGAGAAAGTGTT
TCAACCATGTCAAACAGCTCTTCTTCAAACCATGGTGCATCACCATTATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 26-K104353-0022-764-G06C-8409
GenBank Accession LN497906 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K104353-0022-764-G06C-8409
ATTTACAATTGAATATATAATGGTGATGCACCATGGNTTTGAAGAAGAGCTGTTTGACAT
GGTTGAAACACTTTCTCGCAACAATAATGTCTCCATTTTGCTCAAACAAAGCAATTGCTA
AAAATACTACATTGTATTGTGTTATTTAGCCTTGAATGTTATGCAGTTTTCTACGTTTTG
AAAATGTGATTCCGCTAAATACATACATTTTCTTAAAACTTGTGTTAAATAATAGTACGT
AAAAAATACGTACCAAGTGAATTTTCAGTTTACCATGCACGTTTTAAAATTAAACCAAAA
TTTAAAAATTCCATTCATCAATAGAATAATAGATATTTTTTTTCGGTTTTAAACAAAATT
AATATCTTTTAGCTTAAAATTGTTGTCCCTCTATTGACTACGTAATTTCATAATTCACTT
ATTAATCTGACATACTTACTAGTCAAGCCTTTAGACTAAAATTTTAATACACATTCATAA
TATAAATTTCTGTCTAACTAATCATTACAATCAAAATCTCAAGCATTGAATTGTTGAAAC
TAAGC

Last Updated on 06.12.2018 16:11