Sequences from confirmation amplicon


Confirmation sequences for line 764H08 and hit At5g24780 are shown.
Line ID 764H08
Locus At5g24780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8508085
Primers used for PCR MK34 - 8474, show primer details
   
Sequence Name 04-K031753-022-764-H08C-MK34
GenBank Accession LN487799 [GenBank]
Sequencing primer gene specific primer, MK34: 5'-ATTGCAAAGCCTATGTTGAAGACT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K031753-022-764-H08C-MK34
GTACCATACGACTCCAAACTGTAAACAAAGAGGCATATTTTTACGCCAAAGGACTTGCCC
TAAAGAACGATACCGTCAATGTTTGGATCTTTGACCTAGACGACACTCTCCTCTCTAGTA
TTCCCTACTACGCTAAATATGGATATGGGTATATACATATTATTGTACCTATCTACATTG
AATTAATAATATTATCGTCATATAGTTTTTTTGAAAATGTTTAGCTGTCATATATGGAAA
GACACGTTTCATTTGACAACTTTAAATATTTTAACTAACCCATCTAACTGTTTTTTTGGG
TCCATATCTACTTTGTGATTTCATTCTAATGTAGATTTTTTTTTGTTGGATTCTTAGGAC
CGAGAACACAGCCCCGGGGGCGTACTGGTCGTGGTTAGAGTCCGGAGAATCAACTCCAGG
ACTCCCGGAGACCTTGCATCTATACGAAAACCTCTTGGAACTCGGGATTGAACCCATCAT
AATCTCTGACAGATGGAAAAAATTGTCAGAAGTCACTGTCGAGAATCTACATGGCTATAT
ATAGCAAATAATGTCATATGTGATTGAATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTA
CATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAA
TTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTA
   
Sequence Name 03-K031753-022-764-H08C-8409
GenBank Accession LN487364 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K031753-022-764-H08C-8409
CATTTACAATTGAATATATTCATCACATATGACATTATTTGCTATATATAGCCATGTAGA
TTCTCGACAGTGACTTCTGACAATTTTTTCCATCTGTCAGAGATTATGATGGGTTCAATC
CCGAGTTCCAAGAGGTTTTCGTATAGATGCAAGGTCTCCGGGAGTCCTGGAGTTGATTCT
CCGGACTCTAACCACGACCAGTACGCCCCCGGGGCTGTGTTCTCGGTCCTAAGAATCCAA
CAAAAAAAAATCTACATTAGAATGAAATCACAAAGTAGATATGGACCCAAAAAAACAGTT
AGATGGGTTAGTTAAAATATTTAAAGTTGTCAAATGAAACGTGTCTTTCCATATATGACA
GCTAAACATTTTCAAAAAAACTATATGACGATAATATTATTAATTCAATGTAGATAGGTA
CAATAATATGTATATACCCATATCCATATTTAGCGTAGTAGGGAATACTAGAGAGGAGAG
TGTCGTCTAGGTCAAAGATCCAAACATTGACGGTATCGTTCTTTAGGGCAAGTCCTTTGG
CGTAAAAATATGCCTCTTTGTTTACAGTTTTGGAGTCGTATTGGTACTGTTTGGAAGTGA
TCAAGTAGTCTTCAACATAGCNNTTGCA
Primers used for PCR MK35 - 8474, show primer details
   
Sequence Name 29-K031754-022-764-H08C-8409
GenBank Accession LN499171 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K031754-022-764-H08C-8409
TTACAATTGAATATTTCAAGGCTGTTGGTGTAACAAAATGGAAGCATCTCATACTCAAGT
AAGAATTAATCAAGTAGCTTAGTTATATATAGCAAATAATGTCATATGTGATTGAATATC
GAGGACTATATATGGAAACGCAGGCCAAACGGATCGAAGTTGACGCAAGTGGTGTACAAG
TCAAAGGTTAGGAATAGCCTTGTGAAGAAAGGGTACAACATCGTTGGGAATATTGGAGAC
CAATGGGCTGATTTGGTTGAGGATACTCCTGGAAGGGTTTTTAAGCTCCCAAATCCACTC
TACTACGTACCTTCTTAATTAAGCATCTATCTTCATGGCATTGTCCCCTTGTATCCATTT
CATATCTATGTCGTTTCGTTTATCTTTGTAGCCGTTTTGGCACCACTGCTTAAATAANAT
GCCAA
   
Sequence Name 30-K031754-022-764-H08C-MK35
GenBank Accession LN499604 [GenBank]
Sequencing primer gene specific primer, MK35: 5'-ATTGGCATTTTATTTAAGCAGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K031754-022-764-H08C-MK35
CGAAACGACTAGATATGAAATGGATACAGGGGACAATGCCATGAAGATAGATGCTTAATT
AAGAAGGTACGTAGTAGAGTGGATTTGGGAGCTTAAAAACCCTTCCAGGAGTATCCTCAA
CCAAATCAGCCCATTGGTCTCCAATATTCCCAACGATGTTGTACCCTTTCTTCACAAGGC
TATTCCTAACCTTTGACTTGTACACCACTTGCGTCAACTTCGATCCGTTTGGCCTGCGTT
TCCATATATAGTCCTCGATATTCAATCACATATGACATTATTTGCTATATATAACTAAGC
TACTTGATTAATTCTTACTTGAGTATGAGATGCTTCCATTTTGTTACACCAACAGCCTTG
AAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
GACGTGTCTACATTCACTCCAAAATGGGGAGAAAA

Last Updated on 06.12.2018 16:11