Sequences from confirmation amplicon


Confirmation sequences for line 764H12 and hit At1g80660 are shown.
Line ID 764H12
Locus At1g80660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30318045
Primers used for PCR 47qf - 8474, show primer details
   
Sequence Name 15-K115694-0022-764-H12C-47qf
GenBank Accession LN493113 [GenBank]
Sequencing primer gene specific primer, 47qf: 5'-CCACAAATATGCTTCATCTCTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K115694-0022-764-H12C-47qf
TCAGACAGATGAACAGAGTTAAATTTATGGAGTTGAACCACTTGTGAAGGTAGAAGATTT
GATTGTTTTTACCAGGGAAGACGCCGGCGAAACCATCTGCCTTCTCTATGAGTTCATCAA
CAGGAAGGCTAGCTATAGATTCATCCTTATCTTGTCCAAGTAGAGCAGAAGATGGATACA
TGTTTGTGCCCATCCCAAGCCTACGACCAGTTTCCTTACCAATGGCAAGTTGGTCACCAG
TGATCATCTTGACATTAACACCAAGATCAAGGGCACGCCTGATTGTCTCTGCACTGTCAT
GTCTTGGAGGGTCAAAGAGAGGTAACAGACCAAGAAACTGCCATGGACTATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTATTATA
   
Sequence Name 06-K115693-0022-764-H12C-8409
GenBank Accession LN488951 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K115693-0022-764-H12C-8409
CATTTACATTGAATATAGTCCATGGCAGTTTCTTGGTCTGTTACCTCTCTTTGACCCTCC
AAGACATGACAGTGCAGAGACAATCAGGCGTGCCCTTGATCTTGGTGTTAATGTCAAGAT
GATCACTGGTGACCAACTTGCCATTGGTAAGGAAACTGGTCGTAGGCTTGGGATGGGCAC
AAACATGTATCCATCTTCTGCTCTACTTGGACAAGATAAGGATGAATCTATAGCTAGCCT
TCCTGTTGATGAACTCATAGAGAAGGCAGATGGTTTCGCCGGCGTCTTCCCTGGTAAAAA
CAATCAAATCTTCTACCTTCACAAGTGGTTCAACTCCATAAATTTAACTCTGTTTCATCT
GTCTGATTAGAACACAAGTATGAGATTGTGAAGAGGCTTCAAGAGATGAAGCATATTTGT

Last Updated on 06.12.2018 16:11