Sequences from confirmation amplicon


Confirmation sequences for line 765F08 and hit At1g63050 are shown.
Line ID 765F08
Locus At1g63050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23376273
Primers used for PCR 60by - 8474, show primer details
   
Sequence Name 53-K108225-0022-765-F08C-60by
GenBank Accession LN508179 [GenBank]
Sequencing primer gene specific primer, 60by: 5'-TCCCTAAATCGATATCATGTCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K108225-0022-765-F08C-60by
TTTCCCAAAAATGTCTTTTAGATTAAACAAAGAACAACACTGTGCCGGAGAAGAATCTTG
TTTTGTCACACTTGTTGTTCCGTTAAAAGAAATTCAGATTCGAATAAACTTGACCTCTTT
CAACCAAAAAATATATGAACTTTTTAATCCTTTTTGTTCATTTTCGAGTTGACTTTGGAC
TAGCTTAGATATATATCAGAAAAAATAAAATCATTTTCGAAAATTGGGAAGGAGAAGAAG
AATAAAACCTCAAATCTTGATTTCTCTTGTTTTACATAAAACGGTCCAAGTGGAAAAAAA
GAAAAGAACCAAAATGTTGTTGATTTTTTAAAAAGACAACATTATTCTTCTTTTCTGGTC
TTTGGTCTAACAGGCTTCACAGGAACTAAGTAGCTGAGAAGAAGCACAGCGATAGGTATA
ACTGTTCCAATGTAATATACACTCTTGAAGGCGACTAGTGTTTCGTGCAAGCTTAAAACC
TGAAAAATTACACACAAAAATGAGAGTTTTTTGAAATGAAAACACCGTTGAAAAAAACGG
AGGGAAGAGTTTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 37-K108224-0022-765-F08C-8409
GenBank Accession LN502641 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K108224-0022-765-F08C-8409
TTGAAAAACTCTTCCCTCCGTTTTTTTCAACGGTGTTTTCATTTCAAAAACTCTCATTTT
TGTGTGTAATTTTTCAGGTTTTAAGCTTGCACGAAACACTAGTCGCCTTCAAGAGTGTAT
ATTACATTGGAACAGTTATACCTATCGCTGTGCTTCTTCTCAGCTACTTAGTTCCTGTGA
AGCCTGTTAGACCAAAGACCAGAAAAGAAGAATAATGTTGTCTTTTTAAAAAATCAACAA
CATTTTGGTTCTTTTCTTTTTTTCCACTTGGACCGTTTTATGTAAAACAAGAGAAATCAA
GATTTGAGGTTTTATTCTTCTTCTCCTTCCCAATTTTCGAAAATGATTTTATTTTTTCTG
ATATATATCTAAGCTAGTCCAAAGTCAACTCGAAAATGAACAAAAAGGATTAAAAAGTTC
ATATATTTTTTGGTTGAAAGAGGTCAAGTTTATTCGAATCTGAATTTCTTTTAACGGAAC
AACAAGTGTGACAAAACAAGATTCTTCTCCGGCACAGTGTTGTTCTTTGTTTAATCTAAA
AGACATTTTTTGGGAAATTAATAATATTATCATCTTAAGATTACATTTAGGAGACATGAT
ATCGATTTAGGGA

Last Updated on 06.12.2018 16:11