Sequences from confirmation amplicon


Confirmation sequences for line 766H08 and hit At2g30390 are shown.
Line ID 766H08
Locus At2g30390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12954095
Primers used for PCR NL83 - 8474, show primer details
   
Sequence Name 05-K103931w-0022-766-H08C-NL83
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, NL83: 5'-AAGTAACCAAAGCTGCTCTCAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K103931w-0022-766-H08C-NL83
TTCAGAGCCGGTTTGAGAGTAGCAGCAATGCTTCAAGGGTAATCTTGTGCAGTGCATAAC
AACAGATCTTTGTGAATCGTTACTCAATTGTGGCAAGAGTCTGCATATAACAACAATAAA
CAGAGAACACTAATCAGTTGCAATGTGGAGCTAACAGAGATTATAATCATCATCCAAAAC
TTACGGAGGAGGAGGACGAAACGTTGAGTAGGAAGAAGAAGAGGAAGAAGAAGGAGAAGC
AGTCATGGCTGGGCAATTCATTGCAAAAAGATTGAAACTTGAAGAAACCTTAACGCAACG
ATTTGGATTTGGAGGAGCTAGCTACAAAAATCTCATTTATATTTCTTCGAATTTGTTTTA
TTTTTTCATTAATCAGACCAGACACAGTGGATCAGCAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTA
   
Sequence Name 21-K103932w-0022-766-H08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K103932w-0022-766-H08C-8409
GTCGATAATGAAAAATAAAACAAATTCGAAGAAATATAAATGAGATTTTTGTAGCTAGCT
CCTCCAAATCCAAATCGTTGCGTTAAGGTTTCTTCAAGTTTCAATCTTTTTGCAATGAAT
TGCCCAGCCATGACTGCTTCTCCTTCTTCTTCCTCTTCTTCTTCCTACTCAACGTTTCGT
CCTCCTCCTCCGTAAGTTTTGGATGATGATTATAATCTCTGTTAGCTCCACATTGCAACT
GATTAGTGTTCTCTGTTTATTGTTGTTATATGCAGACTCTTGCCACAATTGAGTAACGAT
TCACAAAGATCTGTTGTTATGCACTGCACAAGATTACCCTTTGAAGCATTTGCTGCTACT
TCATCAAACCGGCTTCTTGGGAAACATTCATTGCCTTTGAGAGCAGCTTTGGTTACT

Last Updated on 06.12.2018 16:11