Sequences from confirmation amplicon


Confirmation sequences for line 767B03 and hit At1g63290 are shown.
Line ID 767B03
Locus At1g63290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23473627
Primers used for PCR 18di - 8474, show primer details
   
Sequence Name 70-K109782-0022-767-B03C-18di
GenBank Accession LN512410 [GenBank]
Sequencing primer gene specific primer, 18di: 5'-GGGCTTTACTTGTACCCAAACATA-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K109782-0022-767-B03C-18di
GTGACTTTCAGCATCAATGATCAATGTTATCATTCATGTGTGTATTTAATATGCCTCTAT
GCATTCATATGCCCAATTAACTCACAAGCATCAATGATCAATGTATCATTCATATGTGTA
TGTAATATGACTCTATGCCTGATGCATTCATATGCTCAATTTCTCTACTAATACCTTTAG
TTGTTGTAGATAAGATGTTCGACAAAATGTGTAAGTGAAGTTCTTAGAAGTGGTTATGTA
TTAGTTTGCCTTGGTGTTTGGTGCCACGTCATCAAGTTTGGTCGTATCAAGCTACCTGGT
TCGGGTTGAGATTCACGTTAACAGGTTGACAAACCTACCCAACGCTCGCTTTTTCTGAGG
GCTTCTATTCTTACAACGCTC
   
Sequence Name 67-K109781-0022-767-B03C-8409
GenBank Accession LN511835 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K109781-0022-767-B03C-8409
ATTTACAATTGAATATATCCTGAGATCACCATCTGCGTTAGGTGAGAGCACTGTGAGAAG
AGAAGAAATCAGAAAAAGCGACCAATGGGTAATTGGGTCAACCCGTTAACCCGAATCTCA
ACCCGACCCAGCTAGCTTGATACGACCAAACCTGATGACGTGGCACCAAACACCAAGGCA
AACTAATACATAACCACTTCTAAGAACTTCACTTACACATTTTGTCGAACATCTTATCTA
CAACAACTAAAGGTATTAGTAGAGAAATTGAGCATATGAATGCATCAGGCATAGAGTCAT
ATTACATACACATATGAATGATACATTGATCATTGATGCTTGTGAGTTAATTGGGCATAT
GAATGCATAGAGTCATATTAAATACACACATGAATGATAACATTGATCATTGATGCTTGA
AAGTTCACACATTACACAGATTTTTTCGTTAATATGTTTGGGTACAAGTAAAGCCCACCA

Last Updated on 06.12.2018 16:11