Sequences from confirmation amplicon


Confirmation sequences for line 767B08 and hit At2g05310 are shown.
Line ID 767B08
Locus At2g05310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:1933415
Primers used for PCR 73ra - 8474, show primer details
   
Sequence Name 48-K115914-0022-767-B08C-73ra
GenBank Accession LN506605 [GenBank]
Sequencing primer gene specific primer, 73ra: 5'-TCTGTTAATCCTTGGTTCTTGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K115914-0022-767-B08C-73ra
TAAGATAGAAGAGTTCATTACCAAGATGAAGAACCAGAACAGTTAGTTCATCTTAATTCT
CAATTTTTTCCATCATGAGATTGTAGAAAATAAACAGAAACTCATTGGATGGTAATGTAA
TTTTAGTCTCCTTTGCAGAATTTAGATTTTGTTTGGTATGTGACAATGACATGTAGACTA
GTGGTTTGTGTTAGTATTGTATCAATACACGAACCATAAATTTGATCGAAAATATTTGGT
TTTTGTAAGTTAGTCAAAAGAATCTTAAACTCTTGCATGCACATTTTTCATAAACATGCA
ATATTGTGACTGACGAAATGGTTATGGATGAGATTTGCATTACAACTTTATAGGTATTGG
CAAAGCGTTGGAGAAAGAGAAGGAGAGAATCCGATGAAGACGCCTCTTCCTTACATTATC
ATCTTCGGTATGTCAACGCCATTCGTCATCTTAGCCATTGCTTTTGCCAATGGTTGGATC
AAAGTTCCCATTCGTATAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGC
   
Sequence Name 22-K115913-0022-767-B08C-8409
GenBank Accession LN496258 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K115913-0022-767-B08C-8409
ACCATTGGCAAAAGCAATGGCTAAGATGACGAATGGCGTTGACATACCGAAGATGATAAT
GTAAGGAAGAGGCGTCTTCATCGGATTCTCTCCTTCTCTTTCTCCAACGCTTTGCCAATA
CCTATAAAGTTGTAATGCAAATCTCATCCATAACCATTTCGTCAGTCACAATATTGCATG
TTTATGAAAAATGTGCATGCAAGAGTTTAAGATTCTTTTGACTAACTTACAAAAACCAAA
TATTTTCGATCAAATTTATGGTTCGTGTATTGATACAATACTAACACAAACCACTAGTCT
ACATGTCATTGTCACATACCAAACAAAATCTAAATTCTGCAAAGGAGACTAAAATTACAT
TACCATCCAATGAGTTTCTGTTTATTTTCTACAATCTCATGATGGAAAAAATTGAGAATT
AAGATGAACTAACTGTTCTGGTTCTTCATCTTTGGTAATGAACTTCTTCTTATCTTTAGG
CTTCACTTCTCCATCTCCTCCACCTTAAGCAAGAACCA

Last Updated on 06.12.2018 16:11