Sequences from confirmation amplicon


Confirmation sequences for line 767H04 and hit At1g76040 are shown.
Line ID 767H04
Locus At1g76040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28539928
Primers used for PCR TR11 - 8474, show primer details
   
Sequence Name 69-K027642-022-767-H04C-8409
GenBank Accession LN512114 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K027642-022-767-H04C-8409
TAGGCTGATGTGGACAAAGTGGGACGATTGATTACATTGAGTTTGTAACTGCAACAATGC
ATCGTCATAGATTGGAGAAAGAAGAGAATTTGATAGAAGCATTTAAATACTTCGACAAAG
ACAGAAGCGGGTTTGTATTCTTCTAACCATCTCCTTCTTCACCAAAATGAACACAACTAC
TTTATATATCAAAGATATATGGAATTGTCTTCAAAACATACTCTTCTTTTTTTTTCAAAA
ACCAGTTTTATCACAAGGGACGAGCTTAAACACTCCATGACTGAGTATGGCATGGGTGAT
GATGCGACCATAGATGAAGTTATCAATGATGTTGATACCGACAATGTAAGAAAAAACATT
TATTTCTAGTTGTTTTGTTTCGGTCTAATGATGAATGTTAAACAAAATGATTAATAATGA
TGATGATGATGATTNGATAGGATGGGAGGATAAACTACGAGGAATTTGTGGCGATGATGA
GGAAAGGAACTACAGATTC
   
Sequence Name 70-K027642-022-767-H04C-TR11
GenBank Accession LN512298 [GenBank]
Sequencing primer gene specific primer, TR11: 5'-ATCTGATCAGCTTTGGATCTGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K027642-022-767-H04C-TR11
ATCGCCACAATTCCTCGTAGTTTATCCTCCCATCCTATCAATCATCATCATCATCATTAT
TAATCATTTTGTTTAACATTCATCATTAGACCGAAACAAAACAACTAGAAATAAATGTTT
TTTCTTACATTGTCGGTATCAACATCATTGATAACTTCATCTATGGTCGCATCATCACCC
ATGCCATACTCAGTCATGGAGTGTTTAAGCTCGTCCCTTGTGATAAAACTGGTTTTTGAA
AAAAAAAGAAGAGTATGTTTTGAAGACAATTCCATATATCTTTGATATATAAAGTAGTTG
TGTTCATTTTGGTGAAGAAGGAGATGGTTAGAAGAATACAAACCCGCTTCTGTCTTTGTC
GAAGTATTTAAATGCTTCTATCAAATTCTCTTCTTTCTCCAATCTATGACGATGCATTGT
TGCAGTTACAAACTCAATGTAATCAATCGTCCCACTTTTGTCCACATCAGCCTATGAATA
AAGAAAAACCATTGAGAAAGATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
AAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAA
GCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11