Sequences from confirmation amplicon


Confirmation sequences for line 768A06 and hit At4g34650 are shown.
Line ID 768A06
Locus At4g34650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16544344
Primers used for PCR PF24 - 8474, show primer details
   
Sequence Name 16-K102864-0022-768-A06C-8409
GenBank Accession LN493387 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K102864-0022-768-A06C-8409
GCCATTTACATTGAAATTTCCCGGACATGAAGACATTGATCAATTGCTGATCTATGATTT
TTCTTGCATGCTACAAACAAAGGTATGTATACGTTACTACATTTCCTGTGTATAGTGTTG
GTAACTAGAAGATGAGATTGTATGTGTTTTAATATTAGGTTGACAATAACGATCCAAATG
CTATGAAAACATTAAACCGACTCGAAACCATCAAGAAATTTTGCAAAGAAAATGGAGGAC
TTCACAAAAGGTAGACTGTTTTTTTTTTGGTTTCTTCTAGTTGTTCTATCAAATAAAAGA
AGATCCAAAGTCACTTGACCAATCTTGTCATGTTCATTTATTACGAGAATGATCTTATAT
GTTTTGGTTGAGGTTCTAATGAATCTTTTTCTGTTTTTTCTTCTTAATGCTGGTCAAAAA
ATCTTATGTTAACGATGAAACACAATCCAAGGCTATCTTTGTAAGTTCAAATTTGTATCA
AACTTCCATAGAAATTCTTTCATATATGGGATTAAGTTACTTATCCTCCTCCTCTAATCT
ACTCCTGAAATTGCAGGTTGTAATGTTTGTGCTTCTACGGGCCATAGTCGTTGTATATCT
CAAAGCAAACCA
   
Sequence Name 16-K102869-0022-768-A06C-PF24
GenBank Accession LN493388 [GenBank]
Sequencing primer gene specific primer, PF24: 5'-TCACTTACATTGGTTTGCTTTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K102869-0022-768-A06C-PF24
AACATTACAACCTGCAATTTCAGGAGTAGATTAGAGGAGGAGGATAAGTAACTTAATCCT
ATATATGAAAGAATTTCTATGGAAGTCTGATACAAATCTGAACTTACAAAGATAGCCTTG
GATTGTGTTTCATCGTTAACATAAGATTTTCTGACCAGCATTAAGAAGAAAAAACAGAAA
AAGATTCATTAGAACCTCAACCAAAACATATAAGATCATTCTCGTAATAAATGAACATGA
CAAGATTGGTCAAGTGACTTTGGATCTTCTTTTATTTGATAGAACAACTAGAAGAAACCA
AAAAAAAAACAGTCTACCTTTTGTGAAGTCCTCCATTTTCTTTGCAAAATTTCTTGATGG
TTTCAAGTCGGTTTAATGTTTTCATAGCATTTGGATCGTTATTGTCAACCTAATATTAAA
ACACATACAATCTCATCTTCTAGTTACCAACACTATACACAGGAAATGTAGTAACGTATA
CCTACCTTTGTTTGTAGCAGGCAAGAAAAATCATAGATAAGCAATTGATCAATGTCTTCA
TGTCCGGGAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATCTCCCCACCGTTTTTATTAAATGAAAGTACATTTTGATAAAACGACAA
ATTACGATC

Last Updated on 06.12.2018 16:11