Sequences from confirmation amplicon


Confirmation sequences for line 768D10 and hit At3g05510 are shown.
Line ID 768D10
Locus At3g05510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1596435
Primers used for PCR 21qr - 8474, show primer details
   
Sequence Name 54-K115779-0022-768-D10C-21qr
GenBank Accession LN508584 [GenBank]
Sequencing primer gene specific primer, 21qr: 5'-AACTGGCATTATATCCTGCATACC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K115779-0022-768-D10C-21qr
ATCTGCGTCCAAAATCAACCTAAAACAAATAAAACTTGTTATAGAATAAAATGATAAGAC
ACATTAAAGTGGAGTGCTTTATATAAGGGAAGTGAACCCAAATCACACATGACTCACCTT
CCGATACCCCTCTTCGCTGAGCCCATAGTCTTTCCACCATCCCGGGAGCGACTGCCTTCT
GGAAAGATGTGAACCCACCCTCCGTTGTTCAATTTCGAAATCGCAATGTCCATTCCCTGC
AGAAGATGTGACACTTGTTTTCATCAGTAAATTTGAATATAGATAAAATAGCAGGTAACA
ATCAAGAAAAGCTAGCTACAAAAACAGCCTGTGTTACCTGCTGATAAATTCCTTCACCAC
GAGAAATTGGCAAAACTTTGACAGATCGAGAGAAAGCTGAAGTTACAGGGTTTTTAAAGC
ATCTATCTGTAGCACAGAGCGTCCACCTCAAATTACGGGCATCAAGTAGAAATTTAGGTG
GAAGTAATGAAGCAATGACAAATGGATCATCCACAGATGCAACATGATTGCTCACCTGAA
GATTATTAAGAAGTTCATGGGTAAGAAAACAAGCAGGTTCATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAG
   
Sequence Name 06-K115778-0022-768-D10C-8409
GenBank Accession LN488953 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K115778-0022-768-D10C-8409
CCATGAACTTCTTAATAATCTTCAGGTGAGCAATCATGTTGCATCTGTGGATGATCCATT
TGTCATTGCTTCATTACTTCCACCTAAATTTCTACTTGATGCCCGTAATTTGAGGTGGAC
GCTCTGTGCTACAGATAGATGCTTTAAAAACCCTGTAACTTCAGCTTTCTCTCGATCTGT
CAAAGTTTTGCCAATTTCTCGTGGTGAAGGAATTTATCAGCAGGTAACACAGGCTGTTTT
TGTAGCTAGCTTTTCTTGATTGTTACCTGCTATTTTATCTATATTCAAATTTACTGATGA
AAACAAGTGTCACATCTTCTGCAGGGAATGGACATTGCGATTTCGAAATTGAACAACGGA
GGGTGGGTTCACATCTTTCCAGAAGGCAGTCGCTCCCGGGATGGTGGAAAGACTATGGGC
TCAGCGAAGAGGGGTATCGGAAGGTGAGTCATGTGTGATTTGGGTTCACTTCCCTTATAT
AAAGCACTCCACTTTAATGTGTCTTATCATTTTATTCTATAACAAGTTTTATTTGTTTTA
GGTTGATTTTGGACGCAGATACTCTCCCTATGGTTGTTCCTTTTGTGCATACTGGTATGC
AGGATATAATGCCA

Last Updated on 06.12.2018 16:11