Sequences from confirmation amplicon


Confirmation sequences for line 768H08 and hit At1g51130 are shown.
Line ID 768H08
Locus At1g51130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18939653
Primers used for PCR 74ra - 8474, show primer details
   
Sequence Name 49-K115914-0022-768-H08C-74ra
GenBank Accession LN506949 [GenBank]
Sequencing primer gene specific primer, 74ra: 5'-AAGTCCACTCTCTTGTGTCTGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K115914-0022-768-H08C-74ra
TCCTCAGTGCCGAGAAAGCACCTGAAGCAAACGTGGTGATGTCGGGTGAAGTCATTTATA
ACCATTTTGTGTTTAGATTAGATTTCAAAGATTGGAAGGTAATAAAATAGAATCTCATTT
CGTTCTCTAGATTGTTAAATTATGTCCTGAAGATGTCTGATTTATGTTGATGTGATGATA
TATGTCAAACAGTTGATGTCTGAAATGGTGCCACTGGGCGAGGAGCTAATGCCACACAGA
CAAACTGCAGTTGCTTCATCTTCTTGTCCCGCTGATGAGATCAGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 23-K115913-0022-768-H08C-8409
GenBank Accession LN496696 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K115913-0022-768-H08C-8409
ATCCTGATCTCATCAGCGGGACAAGAAGATGAAGCAACTGCAGTTTGTCTGTGTGGCATT
AGCTCCTCGCCCAGTGGCACCATTTCAGACATCAACTGTTTGACATATATCATCACATCA
ACATAAATCAGACATCTTCAGGACATAATTTAACAATCTAGAGAACGAAATGAGATTCTA
TTTTATTACCTTCCAATCTTTGAAATCTAATCTAAACACAAAATGGTTATAAATGACTTC
ACCCGACATCACCACGTTTGCATCAGGTGCATTTCTCGGCACTGAGGACACATTAGGAGA
TCAAGTATAAGTGAAACGAATTAAATATCAAAACCAGACACAAAGAGTATGGACTTA

Last Updated on 06.12.2018 16:11