Sequences from confirmation amplicon


Confirmation sequences for line 769D03 and hit At4g12620 are shown.
Line ID 769D03
Locus At4g12620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7461859
Primers used for PCR PF73 - 8474, show primer details
   
Sequence Name 18-K102867-0022-769-D03C-8409
GenBank Accession LN494257 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K102867-0022-769-D03C-8409
ATTTACATTGAATATAATAAACTCTCTTCTTCTTCTTCGTCTTCTTAATCGTCTCAGATC
TAATTGGTGAAACAGGAGTGAAGGAATCTATCTTCTTCTTCTTCTTCGGAGTTTCTGTTT
TCTTCGACTTCGATGGAACGACATCAGTGGTTGGTTTTCTGGGGGCACGTTCTCGTTTTC
GAATTAGATTCATTCCTTCGATTGGGTCATTTCCTGGAGCATCAATTGGGTCATTTCCCA
GATCGATTTTACGAGAAACATGACGAGCAGATCTACGGAGAGGAGTGTGAGTTTCTGGGG
TTTGAGGAGTGTGTGAGGTTGAAGATGGAGATAGATACGATTTGCGATAGATGTTAGACG
GAGTTTTGGTTGGGGATTTGAAGGTTTTTGCTCTTGGAGTTGAAGCCATTGATGGTGTAA
GCTAGGGTTTTTCTGGGTGAAGAATGAAGAG
   
Sequence Name 18-K102868-0022-769-D03C-PF73
GenBank Accession LN494258 [GenBank]
Sequencing primer gene specific primer, PF73: 5'-ACTCTTCATTCTTCACCCAGAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K102868-0022-769-D03C-PF73
CATGGCTTCACTCCAGAGCAAAACCTTCAATCCCCAACCAAAACTCCGTCTAACCTCTAA
GGGGAATCGTATCTATCTCCATCTTCAACCTCACACACTCCTCAAACCCCAGAAACTCAC
ACTCCTCTCCGTAGATCTGCTCGTCATGTTTCTCGTAAAATCGATCTGGGAAATGACCCA
ATTGATGCTCCAGGAAATGACCCAATCGAAGGAATGAATCTAATTCGAAAACGAGAACGT
GCCCCCAGAAAACCAACCACTGATGTCGTTCCATCGAAGTCGAAGAAAACAGAAACTCCG
AAGAAGAAGAAGAAGATAGATTCCTTCACTCCTGTTTCACCAATTAGATCTGAGACGATT
AAGAAGACGAAGAAGAAGAAGAGAGTTTATTATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTACATTCACGTCCAAATGG

Last Updated on 06.12.2018 16:11