Sequences from confirmation amplicon


Confirmation sequences for line 769D04 and hit At1g53165 are shown.
Line ID 769D04
Locus At1g53165 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19818782
Primers used for PCR 24wm - 8474, show primer details
   
Sequence Name 48-K118761-0022-769-D04C-24wm
GenBank Accession LN506648 [GenBank]
Sequencing primer gene specific primer, 24wm: 5'-TGGTTATTTCTGACATGAAAGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K118761-0022-769-D04C-24wm
ATCGCGATCTTCGATAACAAGACAGTCATGAGATCACAACAAGTGAGTGATGATGAAGAA
GATGACTCCAAATTGGCATCATTGTCTGCATCATTGTCACTATTGCTATTACCATCCCTA
AAAGAGGTAAGCTATAAACTACGCTCACATTTGCCATCTTACTTCTCTCAATGAGTTATG
AGTCATCTTCAATTTAGAGAAAAAAAAGTTTCGGTCTTGAGTTAGAAGCTGCAAATAATA
TAGGCTGTTGGGGGTGACGACTCAAAAGGAACTATTGGACATAGAGTTTCGAGATCCTTA
GTTAAAATGGAACGCGAAATGGCTTCATGTCCGGGAAATCTACATGAATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATT
   
Sequence Name 23-K118760-0022-769-D04C-8409
GenBank Accession LN496768 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K118760-0022-769-D04C-8409
CATTTCGCGTTCCATTTTAACTAAGGATCTCGAAACTCTATGTCCAATAGTTCCTTTTGA
GTCGTCACCCCCAACAGCCTATATTATTTGCAGCTTCTAACTCAAGACCGAAACTTTTTT
TTCTCTAAATTGAAGATGACTCATAACTCATTGAGAGAAGTAAGATGGCAAATGTGAGCG
TAGTTTATAGCTTACCTCTTTTAGGGATGGTAATAGCAATAGTGACAATGATGCAGACAA
TGATGCCAATTTGGAGTCATCTTCTTCATCATCACTCACTTGTTGTGATCTCATGACTGG
TCTTTGTTTATCAGAATGATCGCGGGAATTTCTGCAAGGTAACAGCTTTCATGTCAGAAA
TAACC

Last Updated on 06.12.2018 16:11