Sequences from confirmation amplicon


Confirmation sequences for line 769F01 and hit At1g61270 are shown.
Line ID 769F01
Locus At1g61270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22601529
Primers used for PCR GE36 - 8474, show primer details
   
Sequence Name 07-K100994-0022-769-F01C-8409
GenBank Accession LN489197 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K100994-0022-769-F01C-8409
CCATTTACATTGAATATACAAACCAAGCAATAGTGTATATGGTGACAGGAGGTGAATCAC
TGAAAAAGATTCATCAACTTTCTGTGGGAGACTACGAGTGTAGAAAGTTGAAAGTTCGAC
ACTTCATTTTGATCTTTGCCTCTTCACAGTTTGTGCTTTCCCTTCTTAAAAATTTCAACT
CCATTTCCGGTGTCTCTTTGGTAGCTGCCGTCATGTCTATGAGGTAAGTGAATCTCATCC
TCTTTGTTTCTATGTATCATGAGCTTGTGCTTTAGATTAACCTCGATAAACATGTGGTCA
GTAATCCAGCCTTAGAACTAGAGAATATATTAGTTTAAGCTTCCTCTTAAGCCAAGTCTC
TCTTATAGAATTAGGTTAGTTGTTGGAAGTTATTTCTTTCATTAAGAGGAAGATACCTTA
TATAGGCCACGATACATTGCATTTAAAGAAATCTTAAAACCTAACGTGAAAAAAGAATAC
TCTCAAAACCAATATTAAAAGGAAAAGACCAAAACACC
   
Sequence Name 07-K100998-0022-769-F01C-GE36
GenBank Accession LN489198 [GenBank]
Sequencing primer gene specific primer, GE36: 5'-TTCTATTAGCGGTGTTTTGGTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K100998-0022-769-F01C-GE36
TCTTTTTTCCGTAGGTTTAAGATTTCTTTAATGCAATGTATCGTGGCCTATATAAGGTAT
CTTCCTCTTAATGAAAGAAATAACTTCCAACAACTAACCTAATTCTATAAGAGAGACTTG
GCTTAAGAGGAAGCTTAAACTAATATATTCTCTAGTTCTAAGGCTGGATTACTGACCACA
TGTTTATCGAGGTTAATCTAAAGCACAAGCTCATGATACATAGAAACAAAGAGGATGAGA
TTCACTTACCTCATAGACATGACGGCAGCTACCAAAGAGACACCGGAAATGGAGTTGAAA
TTTTTAAGAAGGGAAAGCACAAACTGTGAAGAGGCAAAGATCAAAATGAAGTGTCGAACT
TTCAACTTTCTACACTCGTAGTCTCCCACAGAAAGTTGATGAATCTTTTTCAGTGATTCA
CCTCCTGTCACCATATACACTATTGCTTGGTTTGTATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11