Sequences from confirmation amplicon


Confirmation sequences for line 769H12 and hit At5g35770 are shown.
Line ID 769H12
Locus At5g35770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:13940205
Primers used for PCR BD58 - 8474, show primer details
   
Sequence Name 28-K028349-022-769-H12C-8409
GenBank Accession LN498707 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K028349-022-769-H12C-8409
ATATATCCTGAAAAAAAAATTAAAGCCATTCAATTGAATATATCCTGAAGCCATTGGTAA
TATCATATTCACTTAATTATGATCTAAAGAGTAAGAATTCGTTATACCAAAAAGAATTAG
AACGGCAGAATCAAATCTAAGCTTAGAAACAACTAAAGTACAAGACACTTGAAACAAGCA
GTACATTTGACTAGACCAAGATTGTGTCCCTCCACTTAAGTTATTATAGACCCACGACTA
CTTTGACTTTGTTTTGTCTGCATAAGATCTATGTCTCACTATTTTCAAGTTCAAATATAA
GCTAATAAACGAATTCAGATAGTGTAACGAAAATATTACTTTACTACCTGAAGTACATAT
ATATATAGAGAGACACGAGATAATTGATGTGTCTCGACCGTTACAAAACTAAAAGAAGGT
ATTCAAAGAACGTTAGAACTGACCCGAAAAACGTTGGCAAGAGCCGGAGCTGCGGCGAGG
AGGCCGGCGGATGTGGAAGCGTTAACGGCAACTTGAACGGCGAGAGATTCAAGAAAAGGC
TCCGGCCAAACATCGTTGGCTCCGCGGCGAGGGCGGGATGGAGATGGGGCCTCGAAAACG
CCGCCGCTTCCACCGGCTCCGTTGTCGGAAGA
   
Sequence Name 28-K028349-022-769-H12C-BD58
GenBank Accession LN498708 [GenBank]
Sequencing primer gene specific primer, BD58: 5'-ATGTCTACCTCCTCCTCTTCTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K028349-022-769-H12C-BD58
CTCGCCGCGGAGCCAACGATGTTTTGGCCGGAAGCCTTTTTCTTGAATCTCTCGCCGTTC
AAGTTGCCGTTAACGCTTCCACATCCGCCGGCCTCCTCGCCGCAGCTCCGGCTCTTGCCA
ACGTTTTTCGGGTCAGTTCTAACGTTCTTTGAATACCTTCTTTTAGTTTTGTAACGGTCG
AGACACATCAATTATCTCGTGTCTCTCTATATATATATGTACTTCAGGTAGTAAAGTAAT
ATTTTCGTTACACTATCTGAATTCGTTTATTAGCTTATATTTGAACTTGAAAATAGTGAG
ACATAGATCTTATGCAGACAAAACAAAGTCAAAGTAGTCGTGGGTCTATAATAACTTAAG
TGGAGGGACACAATCTTGGTCTAGTCAAATGTACTGCTTGTTTCAAGTGTCTTGTACTTT
AGTTGTTTCTAAGCTTAGATTTGATTCTGCCGTTCTAATTCTTTTTGGTATAACGAATTC
TTACTCTTTAGATCATAATTAAGTGAATATGATATTACCAATGGCTTCAGGATATATTCA
ATTGAATGGCTTTAATTTTTTTTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGT
ACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAA
ATTATTCTAATT

Last Updated on 06.12.2018 16:11