Sequences from confirmation amplicon


Confirmation sequences for line 771C08 and hit At4g37610 are shown.
Line ID 771C08
Locus At4g37610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17672025
Primers used for PCR CP38 - 8474, show primer details
   
Sequence Name 33-K031249-022-771-C08C-8409
GenBank Accession LN500880 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K031249-022-771-C08C-8409
ATTTTTTTTCCCTACCTGTTACAGAAGAGATATGGAGAACATGGACGATTTCTCACCGGA
GAATGTTTTAGCACCACCACCACCACCTCCTCCAATGAAGAAGTCAACGGATCTGTTTAT
GCAAAGAAGCAATAGCTTTGTTTCTAAAGCTACGAGAGATTCATGGGATCGTATGTTCGA
CGAAGCTCATGGAGCTGATGTTTTGATTCATACTGATGATAATGGTTTGATTTACGCACA
TTCTAATGTCATCGTAAGTTCTCTAATCCAATGCTTAGAAAAATCAGTAACATTGTTTTT
TGTATTGAATCTTAATGAAGTTTTATCTGTTAAACTTTAATTAGGGAATGGCTTCAGATG
TAATCAGAGGAATGATGAAGCAACATAAGAGAAAATCTCATCGTAAATCGATTTCAATCC
TCGGTGTTCCTCACCACGCTCTTCGAGTTTTCATTCGATTCTTATACTCTTCTTGGTAAG
TAGCAAAAGTTTTGGATTTTACAAATCTTTGGCTATAAACATTTAGGGATACATACTTAA
ACATTTTGTTTCTTGCTCTGTTTTTGTAGCTATGAGAAACAAGATATGGAGGATTTTGCA
ATACATCTACTTGTGTTATCACATGTCTACGTTGTACCGCATTTGAAGCGAGTCTGCGAA
TCAGAATTCGAAAGTAGTTTGCTCAACAAAGAGAATGTGATTGATGTGTTTCAGCTAGCT
CTTCTATGTGATGCTCCTCGTCTCGGTCTTCTCTGTCATAGAATGATTCTAAACAACTTT
GAAGAAGTTTCTACTTCTGAAGGATGGCAAGCAATGAAAGAGAGCCATCCTAGACTTCAA
AAAGAGCTTTTACGCTCTGTCGCTT
   
Sequence Name 34-K031249-022-771-C08C-CP38
GenBank Accession LN501299 [GenBank]
Sequencing primer gene specific primer, CP38: 5'-TACATTAAGCTCGTAAGCGACAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K031249-022-771-C08C-CP38
AGGCTCTCTTTCATTGCTTGCCATCCTTCAGAAGTAGAAACTTCTTCAAACTTGTTTAGA
ATCATTCTATGACAGAGAAGACCGAGACGAGCAGCATCACATAGAAGAGCTAGCTGAAAC
ACATCAATCACATTCTCTTTGTTGAGCAAACTACTTTCGAATTCTGATTCGCAGACTCGC
TTCAAATGCGGTACAACGTAGACATGTGATAACACAAGTAGATGTATTGCAAAATCCTCC
ATATCTTGTTTCTCATAGCTACAAAAACAGAGCAAGAAACAAAATGTTTAAGTATGTATC
CCTAAATGTTTATAGCCAAAGATTTGTAAAATCCAAAACTTTTGCTACTTACCAAGAAGA
GTATAAGAATCGAATGAAAACTCGAAGAGCGTGGTGAGGAACACCGAGGATTGAAATCGA
TTTACGATGAGATTTTCTCTTATGTTGCTTCATCATTCCTCTGATTACATCTGAAGCCAT
TCCCTAATTAAAGTTTAACAGATAAAACTTCATTAAGATTCAATACAAAAAACAATGTTA
CTGATTTTTCTAAGCATTGGATTAGAGAACTTACGATGACATTAGAATGTGCGTAAATCA
AACCATTATCATCAGTATGAATCAAAACATCAGCTCCATGAGCTTCGTCGAACATACGAT
CCCATGAATCTCTCGTAGCTTTAGAAACAAAGCTATTGCTTCTTTGCATAAACAGATCCG
TTGACTTCTTCATTGGAGGAGGTGGTGGTGGTGGTGCTAAAACATTCTCCGGTGAGAAAT
CGTCCATGTTCTCCATATCTCTTCTGTAACAGGTTAGGGTAAAAAAAATCAGATTCAGAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAAGAGTATTACC
NATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATAATAAAATGAAAGTACAT
TTTGATAAAACGACAAAT

Last Updated on 06.12.2018 16:11