Sequences from confirmation amplicon


Confirmation sequences for line 771C08 and hit At5g58140 are shown.
Line ID 771C08
Locus At5g58140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23533028
Primers used for PCR 46gz - 8474, show primer details
   
Sequence Name 58-K112176-0022-771-C08C-46gz
GenBank Accession LN509746 [GenBank]
Sequencing primer gene specific primer, 46gz: 5'-AAGCTAGAGAGATGATAAGCGTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K112176-0022-771-C08C-46gz
AGAAGAACGGAGAGATCCCATAGAGACAACCTCATAACTTGGTGGTGTCTGGGACTGGAG
AAAGTTGGATGGGAAAGCTCAGAAAGAAGACAGACTGTGTGAAAAGAAGAAAAGACATAA
TGGAATCACGGGGCATAGAGGTGATGATAGTTTAGAATACATTGAAATCCCTTTCACGTG
CACAGCTTCAAAAAAAATCAGAACTTTTAGTCAGAAAGCTATGAAATTTCAGAAGAAGAA
GATAAGAGTTGAATAGTTTGGGGGAGTTCCGGAGTTGAGAGTTGAGTTTTTGGTTGTATC
GTATATGGACACTGGAGAAAGTGGGAAGAGAAGAGAGAGTCGTTGAAAGTCAAAAAAAGG
GATATGTGGAGATTATAATTAGGTAAAGTATTTCGCTAGTGAGTTATAAGAAAAATATGA
TAAATGGAATCTGTCTCAGACCTTTTGTGAATTTTTCCAAATCCCAAAGTTGTCAATGGA
TTTGGTGAGTGGAGGAGCAATTGTAAAAATTCAAAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATTC
   
Sequence Name 42-K112175-0022-771-C08C-8409
GenBank Accession LN504510 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K112175-0022-771-C08C-8409
ACTCACCAAATCCATTGACAACTTTGGGATTTGGAAAAATTCACAAAAGGTCTGAGACAG
ATTCCATTTATCATATTTTTCTTATAACTCACTAGCGAAATACTTTACCTAATTATAATC
TCCACATATCCCTTTTTTTGACTTTCAACGACTCTCTCTTCTCTTCCCACTTTCTCCAGT
GTCCATATACGATACAACCAAAAACTCAACTCTCAACTCCGGAACTCCCCCAAACTATTC
AACTCTTATCTTCTTCTTCTGAAATTTCATAGCTTTCTGACTAAAAGTTCTGATTTTTTT
TGAAGCTGTGCACGTGAAAGGGATTTCAATGTATTCTAAACTATCATCACCTCTATGCCC
CGTGATTCCATTATGTCTTTTCTTCTTTTCACACAGTCTGTCTTCTTTCTGAGCTTTCCC
ATCCAACTTTCTCCAGTCCCAGACACCACCAAGTTATGAGGTTGTCTCTATGGGGATCTC
TCCTGTTCTTCTCCTTCTTCGTCAAGCATCTGACGAGCCTAGATCCCAACACCGACGC

Last Updated on 06.12.2018 16:11