Sequences from confirmation amplicon


Confirmation sequences for line 771D10 and hit At4g32490 are shown.
Line ID 771D10
Locus At4g32490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15679493
Primers used for PCR NA46 - 8474, show primer details
   
Sequence Name 14-K101562-0022-771-D10C-8409
GenBank Accession LN492444 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K101562-0022-771-D10C-8409
CCATTTACATTAAACCCCAAACCCATCAACATCACAATCATCAAGTAAACATCAGTCATT
TTGACAAAAACCATCAATATCCGAACCAAAACTTTAATTAACACCAACTTTCTACTCTCT
TATAGCTTAACTCTCATTAATTTGGTGTGGTTTGGGTGCTTGCTTGTATGTGTATTTATG
GTGAAAGTAGACAAGAGATTTATAGCCGTTGGGTGAAGCGTTTGGCTGTGCATCGATATG
TAGAGAAAATGCCAATTAAGTAGAAGCGTTTCGTTCTTGCTCATCTCTTTCTTTTGTTAC
ACGTTTCGACATGCAATAGACTTAACGGCTATCTACACAATATTACTAGTCAGGTCTTTA
ACTACTCATCATCATTCATCAAGTACAACTTAAAATTTCATTTTTTAAAATTCAAACTTT
AATAACGTATTGTCTTATTTTACACTATTAGCAGGAGATCAGGGA
   
Sequence Name 14-K101571-0022-771-D10C-NA46
GenBank Accession LN492445 [GenBank]
Sequencing primer gene specific primer, NA46: 5'-TCCCTGATCTCCTGCTAATAGTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K101571-0022-771-D10C-NA46
TTAGTTTGATTTTAAAATGAAATTTTAAGTTGTACTTGATGAATGATGATGAGTAGTTAA
AGACCTGACTAGTAATATTGTGTAGATAGCCGTTAAGTCTATTGCATGTCGAAACGTGTA
ACAAAAGAAAGAGATGAGCAAGAACGAAACGCTTCTACTTAATTGGCATTTTCTCTACAT
ATCGATGCACAGCCAAACGCTTCACCCAACGGCTATAAATCTCTTGTCTACTTTCACCAT
AAATACACATACAAGCAAGCACCCAAACCACACCAAATTAATGAGAGTTAAGCTATAAGA
GAGTAGAAAGTTGGTGTTAATTAAAGTTTTGGTTCGGATATTGATGGTTTTTGTCAAAAT
GACTGATGTTTACTTGATGATTGTGATGTTGATGGGTTTGGGGTTTAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11