Sequences from confirmation amplicon


Confirmation sequences for line 772A03 and hit At5g24640 are shown.
Line ID 772A03
Locus At5g24640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8436757
Primers used for PCR NL89 - 8474, show primer details
   
Sequence Name 38-K103934w-0022-772-A03C-NL89
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, NL89: 5'-ACATTAGCCCAGATATGCCAATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K103934w-0022-772-A03C-NL89
TAAAAAGGCCGCAAAGAATATAATATTAATTTCTTGGTGGACAAGCATGTTTGACCATTC
TCATTTTCTTGTGCCCAAACTTATATTTGGTATGCTCCTCCTACATATAGACAAGACAAT
GGTAATTCGACAATTCATCAAGTCTCAAATCTAAATAAGTAAACATGAAGAGTGAAGGTC
GTCATCAAGGCGTCGTAAGGACAGGAATGATTCATCCGCGGGGCTTTAATCCTCGACACA
CCAACCGACTCGATTCACCACCAGTTTTTGGAGATTTTACCAAAATGCAACCAAAGACTA
CCAACCATTCAAATATCATCGGAGAAAGTGAGCGGTCCAAGTACAGTAATTGTCATGTGT
CGCCGGCTATCAAGTCGGGCCATAAATCTAAGGGTCGCCGTAAGCTACAACCGACAAGTT
GGTGGAGTGAGGATAAGCTAGACCGGCTCATCGGGTCGGATTCATCCTCTGCTAAGGATA
TTTTGGATATGTTATGTAGTGAAGATCATGATGAGTATTAATAATTATCAATTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 49-K103935w-0022-772-A03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K103935w-0022-772-A03C-8409
TATACTCATCATGATCTTCACTACATAACATATCCAAAATATCCTTAGCAGAGGATGAAT
CCGACCCGATGAGCCGGTCTAGCTTATCCTCACTCCACCAACTTGTCGGTTGTAGCTTAC
GGCGACCCTTAGATTTATGGCCCGACTTGATAGCCGGCGACACATGACAATTACTGTACT
TGGACCGCTCACTTTCTCCGATGATATTTGAATGGTTGGTAGTCTTTGGTTGCATTTTGG
TAAAATCTCCAAAAACTGGTGGTGAATCGAGTCGGTTGGTGTGTCGAGGATTAAAGCCCC
GCGGATGAATCATTCCTGTCCTTACGACGCCTTGATGACGACCTTCACTCTTCATGTTTA
CTTATTTAGATTTGAGACTTGATGAATTGTCGAATTACCATTGTCTTGTCTATATGTAGG
AGGAGCATACCAAATATAAGTTTGGGCACAAGAAAATGAGAATGGTCAAACATGCTTGTC
CACCAAGAAATTAATATTATATTCTTTGCGTGCCTTTTTTTATGTCGTCACTCGTCATTA
TCTCTACTCTATTGGCATATCTGGGCTAATG

Last Updated on 06.12.2018 16:11