Sequences from confirmation amplicon


Confirmation sequences for line 772C09 and hit At5g18130 are shown.
Line ID 772C09
Locus At5g18130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5995521
Primers used for PCR CS38 - 8474, show primer details
   
Sequence Name 37-K105920-0022-772-C09C-CS38
GenBank Accession LN502618 [GenBank]
Sequencing primer gene specific primer, CS38: 5'-TGAGTCAGAGTGTCTCTCATCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K105920-0022-772-C09C-CS38
GATCTATTTTCATGTCGGCAAATTCGGTTTCCTTCGATTTCTTGAAGGTTATTCTCGGCG
ACGGAAAAGACTCCGTCAAAAGTTCCGGGTCTGGTTCCGAGAAATCCGGATCCAGGTACT
TATTGTGATCTTTGTCGTCCACGGGGGACAAAGGTTCCCAAGTTATTGGAATCTGGAGGA
GTTGTGTGGGGACCACGCTAGATTTCTGATGAAGAAACTCTGTCTTTCCGACAGAATCTT
GAATTGGGCAGATGAAGTAATCACTGTCGAAGCTTTTTGCGGTGTTTCTCATCATCTTCG
TAACTTCATGATCCTCTTCATGACCGAGATCAAGTTCGAGACCCTTGTAGTTGATTTTGG
GGAGTATTTCCCAATCTTCTAGATCCATCTCCTTCGTGTTGTTGTCGAATGATTTCTCGG
ATTAGGGCTTTTGAAAAGTTTTGTTTGGGTGAGAGTGATTTGTTGCAATTATTGGAGCTA
ATTTGTGTTTGGTAATTTTGATTTGTGTTTCATTGAATATACATGGATTTTTGTGATGAA
TCCATGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCG
   
Sequence Name 37-K105921-0022-772-C09C-8409
GenBank Accession LN502619 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K105921-0022-772-C09C-8409
GCCATTTACAATTGAATATACATGGATTCATCACAAAAATCCATGTATATTCAATGAAAC
ACAAATCAAAATTACCAAACACAAATTAGCTCCAATAATTGCAACAAATCACTCTCACCC
AAACAAAACTTTTCAAAAGCCCTAATCCGAGAAATCATTCGACAACAACACGAAGGAGAT
GGATCTAGAAGATTGGGAAATACTCCCCAAAATCAACTACAAGGGTCTCGAACTTGATCT
CGGTCATGAAGAGGATCATGAAGTTACGAAGATGATGAGAAACACCGCAAAAAGCTTCGA
CAGTGATTACTTCATCTGCCCAATTCAAGATTCTGTCGGAAAGACAGAGTTTCTTCATCA
GAAATCTAGCGTGGTCCCCACACAACTCCTCCAGATTCCAATAACTTGGGAACCTTTGTC
CCCCGTGGACGACAAAGATCACAATAAGTACCTGGATCCGGATTTCTCGGAACCAGACCC
GGAACTTTTGACGGAGTCTTTTCCGTCGCCGAGAATAACCTTCAAGAAATCGAAGGAAAC
CGAATTTGCCGACATGAAAATAGATTCACCAGCAGCGAGGTTCACTAGTCCTCTGCCGCA
GAACGATGAGAGACCCCTTGACTC

Last Updated on 06.12.2018 16:11