Sequences from confirmation amplicon


Confirmation sequences for line 772E12 and hit At5g20910 are shown.
Line ID 772E12
Locus At5g20910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7093309
Primers used for PCR 48tv - 8474, show primer details
   
Sequence Name 58-K117029-0022-772-E12C-48tv
GenBank Accession LN509811 [GenBank]
Sequencing primer gene specific primer, 48tv: 5'-TTCTCTCTCTCTTTCCAGTTTTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K117029-0022-772-E12C-48tv
AAACAATATGAGCATAAGCTTTATGTTCTTACTTACTAGTAAGCTTGAAAGAATGGGATT
TTTTTTTTTATACCACCCAAGGCTTTAGGCAAGGAGGGTGAAATGTGTGCTTGCATGGCA
ATTCCTGCATCTTGTCCCCAATAACTAAATTCTCCTTGCAAATGCAACATTCTGCCTCTG
CTCCAAACTTTTTAAGCAATTCCTCGGTGAAAATAATGACGGGGAGTTTCTCTACAACTT
CTTTACTTGCCGGTGGAGCTCTTGGTGGTCCACCATCATCTAGTATCTGCATAAAACACA
TCAAATATCATTTTTCAATCATGAAGCTAAAAAAAAGACCCTCCCAATACAATATTAAAG
GTTGAGGTTAAACACTCTTGATACAAATAAGTGAAACAACCAAGTGGAGAAACTCAATAT
GCAAAAAACAACATATGATTAAAATGCGGATGATTAAAAATTGCTTTCATCTCTATCTTT
AGGTGAAAAAACTTGTGATATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCACCAATGAGTATGATGGTCAATATGGAGAAAAAAAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTA
   
Sequence Name 26-K117028-0022-772-E12C-8409
GenBank Accession LN498098 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K117028-0022-772-E12C-8409
TCTTCCACCTAAAGATAGAGATGAAAGCAATTTCTTAATCATCCGCATTCTACTCATATG
TTGTTTTTTGCATATTGAGTTTCTCCACTTGGTTGTCTCACTCATCTGTATCAAGAGTGT
TTAACCTCAACCTCTAATATTGTATTGGTAGCGTCTTTTTCTTAGCTTCATGATTGAAAA
GTGATATATGATGTGTTCTATGCAGATACTAGATGATGGTGGACCACCAAGAGCTCCACC
GGCAAGTAAAGAAGTTGTAGAGAAACTCCCAGTCATTATTTTCACCGAGGAATTGCTTAA
AAAGTTTGGAGCAGAGGCAGAATGTTGCATCTGCAAGGAGAATCTAGTTATTGGCGACAA
GATGCAGGAATTGCCATGCAAGCACACATTTCACCCTCCTTGCCTAAAGCCTTGGCTGGT
ATAAAAAAAAAAATCCCATTCTTTCAAGCTTACTAGTAAGTAAAAACATAAAGCTTATGC
TCATATTGTTTTATGGCAGGACAAGCATAACTCTTGCCCTATATGCCGCCTTGAATTACC
AACAGACGATCAGAATTACGAAAACTGG

Last Updated on 06.12.2018 16:11